DNA Fingerprinting of Vegetable Soybean Cultivar 'Zhexian No.9' using 101 New Developed HRM-Based SNP Markers
文献类型: 外文期刊
第一作者: Fu, X. J.
作者: Fu, X. J.;Pei, J. X.;Zheng, Yt;Guo, D. D.;Yang, Q. H.;Jin, H. X.;Zhu, D. H.;Dong, O. K.;Xu, S. C.
作者机构:
关键词: HRM; SNP fingerprint; Soybean; Zhexian No.9
期刊名称:LEGUME RESEARCH ( 影响因子:0.589; 五年影响因子:0.648 )
ISSN: 0250-5371
年卷期: 2020 年 43 卷 1 期
页码:
收录情况: SCI
摘要: Single nucleotide polymorphisms (SNPs) have been proved to be powerful markers in genetic analysis due to their high abundance and polymorphism in plant genomes. The recently developed high-resolution melting (HRM) analysis method provides a novel, quick, and close-tube PCR approach to analyze SNP variations. In present study, 101 HRM-based SNP markers from 20 soybean chromosomes were developed for genotyping vegetable soybean cultivar 'Zhexian No.9' with 'Williams 82' as reference. 33.7% of these markers were polymorphic between 'Zhexian No.9' and 'Williams 82'. Polymorphic markers were found on 85% (17 of 20) of the soybean chromosomes when comparing 'Zhexian No. 9' and 'Williams 82'. Finally, an array of 101 in-sequence nucleotide letters was generated as the first precise SNP fingerprint of 'Zhexian No.9'. The described marker-developing methodology could be used in other crops with known genomic information.
分类号:
- 相关文献
作者其他论文 更多>>
-
Inheritance and fine mapping of a restorer-of-fertility (Rf) gene for the cytoplasmic male sterility in soybean
作者:Dong, D. K.;Yuan, F. J.;Zhu, S. L.;Yang, Q. H.;Fu, X. J.;Yu, X. M.;Li, B. Q.;Zhu, D. H.;Li, Z.;Yu, W.;Chen, P.
关键词:Glycine max;Cytoplasmic male sterility;Restorer-of-fertility;Genetic mapping
-
Molecular cloning of a new wheat calreticulin gene TaCRT1 and expression analysis in plant defense responses and abiotic stress resistance
作者:Lin, R. M.;Wang, F. T.;Feng, J.;Xu, S. C.;An, Y. Q.;Xu, Y. F.
关键词:Wheat;Calreticulin;Puccinia striiformis;Defense response;Abiotic stress resistance
-
Quantitative trait loci for temperature-sensitive resistance to Puccinia striiformis f. sp tritici in wheat cultivar Flinor
作者:Zhang, Z. Y.;Lin, R. M.;Xu, S. C.;Zuo, L. L.;Cao, Y. Y.
关键词:Simple sequence repeats (SSR);Temperature-sensitive resistance;Wheat yellow rust
-
Race Dynamics, Diversity, and Virulence Evolution in Puccinia striiformis f. sp tritici, the Causal Agent of Wheat Stripe Rust in China from 2003 to 2007
作者:Wu, L. R.;Liu, T. G.;Xu, S. C.;Jin, S. L.;Peng, Y. L.;Wang, B. T.
关键词:
-
Characterization and validation of QTLs for adult plant stripe rust resistance in Chinese wheat landrace Dabaimai
作者:Wang, M. Y.;Xu, M. R.;Wang, F. T.;Feng, J.;Lin, R. M.;Xu, S. C.;Shah, S. J. A.
关键词:Wheat stripe; yellow rust; Puccinia striiformisf; sp; tritici; Resistance; SSR; Gene mapping
-
Inheritance of Stripe Rust Resistance to Predominant Chinese Races in Six Spring Wheat Cultivars from the Pacific Northwest of the United States
作者:Sun, Q.;Lin, F.;Cui, N.;Bai, Y. L.;Xu, X. D.;Feng, J.;Xu, S. C.;Gao, Y.;Wang, R.;Chen, X. M.;Chen, X. M.
关键词:CYR32;CYR33;disease resistance;crop production
-
Identification, Gene Postulation and Molecular Tagging of a Stripe Rust Resistance Gene in Synthetic Wheat CI142
作者:Wang, L. M.;Zhang, Z. Y.;Liu, H. J.;He, M. Z.;Liu, H. X.;Xin, Z. Y.;Xu, S. C.;Veisz, O.
关键词:molecular tagging;wheat breeding program;gene postulation