DNA Fingerprinting of Vegetable Soybean Cultivar 'Zhexian No.9' using 101 New Developed HRM-Based SNP Markers

文献类型: 外文期刊

第一作者: Fu, X. J.

作者: Fu, X. J.;Pei, J. X.;Zheng, Yt;Guo, D. D.;Yang, Q. H.;Jin, H. X.;Zhu, D. H.;Dong, O. K.;Xu, S. C.

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关键词: HRM; SNP fingerprint; Soybean; Zhexian No.9

期刊名称:LEGUME RESEARCH ( 影响因子:0.589; 五年影响因子:0.648 )

ISSN: 0250-5371

年卷期: 2020 年 43 卷 1 期

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收录情况: SCI

摘要: Single nucleotide polymorphisms (SNPs) have been proved to be powerful markers in genetic analysis due to their high abundance and polymorphism in plant genomes. The recently developed high-resolution melting (HRM) analysis method provides a novel, quick, and close-tube PCR approach to analyze SNP variations. In present study, 101 HRM-based SNP markers from 20 soybean chromosomes were developed for genotyping vegetable soybean cultivar 'Zhexian No.9' with 'Williams 82' as reference. 33.7% of these markers were polymorphic between 'Zhexian No.9' and 'Williams 82'. Polymorphic markers were found on 85% (17 of 20) of the soybean chromosomes when comparing 'Zhexian No. 9' and 'Williams 82'. Finally, an array of 101 in-sequence nucleotide letters was generated as the first precise SNP fingerprint of 'Zhexian No.9'. The described marker-developing methodology could be used in other crops with known genomic information.

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