Open-Bud Duplicate Loci Are Identified as MML10s, Orthologs of MIXTA-Like Genes on Homologous Chromosomes of Allotetraploid Cotton

文献类型： 外文期刊

第一作者： Chen, Wei

作者： Chen, Wei;Yao, Jinbo;Li, Yan;Zhu, Shouhong;Guo, Yan;Fang, Shengtao;Zhao, Lanjie;Wang, Junyi;Yuan, Li;Zhang, Yongshan;Lu, Youjun

作者机构：

关键词： open-bud; MIXTA-like ortholog; MYB gene; map-based cloning; cotton (Gossypium spp; )

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： The open-bud (ob) mutants in cotton display abnormal flower buds with the stigma and upper anthers exposed before blooming. This characteristic is potentially useful for the efficient production of hybrid seeds. The recessive inheritance pattern of the ob phenotype in allotetraploid cotton is determined by duplicated recessive loci (ob1ob1ob2ob2). In this study, ob1, which is a MIXTA-like MYB gene on chromosome D13 (MML10_Dt), was identified by map-based cloning. In Gossypium barbadense (Gb) acc. 3-79, a single nucleotide polymorphism (SNP) (G/A) at the splice site of the first intron and an 8-bp deletion in the third exon of MML10_Dt were found, which are the causative mutations at the ob1 loci. A 1783-bp deletion that leads to the loss of the third exon and accounts for the causal variation at the ob2 loci was found in MML10_At of Gossypium hirsutum (Gh) acc. TM-1. The ob phenotype results from the combination of these two loss-of-function loci. Genotyping assays showed that the ob1 and ob2 loci appeared after the formation of allotetraploid cotton and were specific for Gb and Gh, respectively. All Gb lines and most Gh cultivars carry the single corresponding mutant alleles. Genome-wide transcriptome analysis showed that some of the MYB genes and genes related to cell wall biogenesis, trichome differentiation, cytokinin signal transduction, and cell division were repressed in the ob mutants, which may lead to suppression of petal growth. These findings should be of value for breeding superior ob lines in cotton.

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