文献类型： 外文期刊

第一作者： Hu Xuan

作者： Hu Xuan;Wang Dan;Pang Yuxin;Huan Hengfu;Chen Zhijian;Wu Zhisheng;Li Weidong

作者机构：

关键词： Lonicera; Polyploidy; Chromatography, high pressure liquid; Dermatoglyphics; Phytochemicals; Qualitative research

期刊名称：JOURNAL OF TRADITIONAL CHINESE MEDICINE （ 影响因子：0.848； 五年影响因子：1.32 ）

ISSN： 0255-2922

年卷期： 2020 年 40 卷 1 期

页码：

收录情况： SCI

摘要： OBJECTIVE: To develop the chromatographic fingerprint of Lonicera japonica (L. japonica) and evaluate the effects of polyploidy on the quality of L. japonica. METHODS: High-performance liquid chromatography (HPLC) methods used to establish the chromatographic fingerprint were developed. The quality of 11 batches of diploid L. japonica and 13 batches of tetraploid L. japonica collected from different regions across China were analyzed. The contents of five active compounds, consisting of chlorogenic acid, rutin, galuteolin, isochlorogenic acid A and quercetin, were further detected in L. japonica. RESULTS: The chromatographic fingerprint established by the optimized HPLC method was verified for qualitative analysis of L. japonica. Quantitative analysis showed that the contents of chlorogenic acid, isochlorogenic acid A, and quercetin in tetraploid plants were higher than those in diploid plants, whereas, rutin and galuteolin contents in tetraploid plants were lower than those in diploid plants. CONCLUSION: The developed HPLC method is suitable for qualitative analysis of L. japonica. Polyploidy was indicated to influence the chemical properties of L. japonica. Tetraploid L. japonica shows potential for utilization as a medicinal plant with different active components. (C) 2020 JTCM. All rights reserved.

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