Comparative analysis of oligosaccharides in Guanzhong and Saanen goat milk by using LC-MS/MS

文献类型： 外文期刊

第一作者： Lu, Jing

作者： Lu, Jing;Zhang, Yan;Song, Bo;Zhang, Shuwen;Pang, Xiaoyang;Sari, Ratna Nurmalita;Liu, Liu;Lv, Jiaping;Zhang, Yan;Wang, Junhui

作者机构：

关键词： Guanzhong goat; Oligosaccharides; Mass spectrometry; Quantification

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

ISSN： 0144-8617

年卷期： 2020 年 235 卷

页码：

收录情况： SCI

摘要： Human milk oligosaccharides play an important role in promoting healthy growth of infants. Goat milk was one of the alternative sources for producing oligosaccharides. An in-depth understanding the composition and the quantity of oligosaccharides in goat milk was needed for its better utilization. In the present study, oligosaccharides were identified and quantified by using UPLC-MS/MS. The elution condition of UPLC was optimized leading to successful identification of 64 oligosaccharides in goat milk. Furthermore, the method to absolutely quantify 6 oligosaccharides in goat milk had been developed. The oligosaccharides in Guanzhong, local breed in China and Saanen goat milk, were compared by using this method. Five oligosaccharides were significantly different between two breeds. The amount of 6'-sialyllactose was 3.3 times higher in Guanzhong goat milk than that in Saanen goat milk. Guanzhong goat milk could be a potential good source for producing sialylated oligosaccharides, especially 6'-sialyllactose.

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