An increase in cell membrane permeability in the in situ extractive fermentation improves the production of antroquinonol from Antrodia camphorata S-29

文献类型： 外文期刊

第一作者： Liu, Xiao-Feng

作者： Liu, Xiao-Feng;Xia, Yong-Jun;Lai, Phoency F-H;Zhang, Yao;Yi, Zhen-Wei;Xie, Chun-Liang;Ai, Lian-Zhong;Xie, Chun-Liang;Hong, Yi-Qiu

作者机构：

关键词： Antrodia camphorata; Antroquinonol; In situ extractive fermentation; Cell membrane permeability; Fatty acid metabolism

期刊名称：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.346； 五年影响因子：3.426 ）

ISSN： 1367-5435

年卷期： 2020 年 47 卷 2 期

页码：

收录情况： SCI

摘要： The goals of this study were to increase the production of antroquinonol (AQ) and to elucidate the response mechanism of the cell membrane during the in situ extractive fermentation (ISEF) of Antrodia camphorata S-29. Through ISEF, the concentration of AQ reached a maximum of 146.1 +/- 2.8 mg/L, which was approximately (7.4 +/- 0.1)-fold that of the control (coenzyme Q(0)-induced fermentation). Transcriptome sequencing showed that four genes (FAD2, fabG, SCD, and FAS1) related to fatty acid biosynthesis were upregulated. FAD2 and SCD may regulate the increase in oleic acid (C18:1) and linoleic acid (C18:2) in the cell membrane of A. camphorata S-29, resulting in an increase in cell membrane permeability. AQ was successfully transferred to the n-tetradecane phase through the cell membrane, reducing product feedback inhibition and improving the production of AQ from A. camphorata S-29.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序