Effect of season on the in-vitro maturation and developmental competence of buffalo oocytes after somatic cell nuclear transfer

文献类型： 外文期刊

第一作者： Zheng, Hai-Ying

作者： Zheng, Hai-Ying;Yang, Chun-Yan;Yu, Nong-Qi;Huang, Jia-Xiang;Zheng, Wei;Abdelnour, Sameh A.;Shang, Jiang-Hua;Abdelnour, Sameh A.;Abdelnour, Sameh A.

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关键词： Season; Oocytes; Maturation; Nuclear transfer; Buffalo

期刊名称：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH （ 影响因子：4.223； 五年影响因子：4.306 ）

ISSN： 0944-1344

年卷期： 2020 年 27 卷 7 期

页码：

收录情况： SCI

摘要： Somatic cell nuclear transfer (SCNT) is a valuable technology tool with various uses in transgenic animals, regenerative medicine, and stem cell research. However, the efficiency of SCNT embryos appears to have poor developmental competency. Environmental issues may adversely affect SCNT embryos in buffalo. Thereafter, the present study aimed to explore the effect of season on the maturation of buffalo oocytes and subsequent developmental capability after parthenogenetic activation and SCNT in buffalo. Buffalo oocytes (n = 6353) were collected from local slaughterhouse at various seasons; spring (March-April), summer (May-August), autumn (September-November), and winter (December-January). A significant increase (p < 0.05) was recorded in the maturation rate (57.07%) at autumn compared with spring, summer, and winter (50.46, 50.93, and 50.66%, respectively). No significant differences were recorded in the fusion and the cleavage rates among all seasons. Blastocyst development rate was higher (p < 0.05) in autumn and winter (16.52 +/- 8.45% and 15.98 +/- 7.17%, respectively) than in spring and summer (9.47 +/- 6.71% and 10.84 +/- 6.58%, respectively) seasons. It could be concluded that the season had a significant effect on oocyte development competence which can be used for SCNT in buffalo.

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