Genetic modification of the protozoan Eimeria tenella using the CRISPR/Cas9 system

文献类型： 外文期刊

第一作者： Tang, Xinming

作者： Tang, Xinming;Liang, Lin;Cui, Shangjin;Suo, Jingxia;Duan, Chunhui;Hu, Dandan;Gu, Xiaolong;Yu, Yonglan;Liu, Xianyong;Suo, Xun;Suo, Jingxia;Duan, Chunhui;Hu, Dandan;Gu, Xiaolong;Yu, Yonglan;Liu, Xianyong;Suo, Xun;Cui, Shangjin

作者机构：

期刊名称：VETERINARY RESEARCH （ 影响因子：3.683； 五年影响因子：4.106 ）

ISSN： 0928-4249

年卷期： 2020 年 51 卷 1 期

页码：

收录情况： SCI

摘要： Eimeria tenella has emerged as valuable model organism for studying the biology and immunology of protozoan parasites with the establishment of the reverse genetic manipulation platform. In this report, we described the application of CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (endonuclease) system for efficient genetic editing in E. tenella, and showed that the CRISPR/Cas9 system mediates site-specific double-strand DNA breaks with a single guide RNA. Using this system, we successfully tagged the endogenous microneme protein 2 (EtMic2) by inserting the red fluorescent protein into the C-terminal of EtMic2. Our results extended the utility of the CRISPR/Cas9-mediated genetic modification system to E. tenella, and opened a new avenue for targeted investigation of gene functions in apicomplexan parasites.

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