Effects of different ATP contents on phosphorylation level of glycogen phosphorylase and its activity in lamb during incubation at 4 celcius in vitro

文献类型： 外文期刊

第一作者： Bai, Yuqiang

作者： Bai, Yuqiang;Li, Xin;Zhang, Dequan;Hou, Chengli;Zheng, Xiaochun;Chen, Li;Ren, Chi

作者机构：

关键词： Activity; ATP; glycogen phosphorylase; lamb; protein phosphorylation

期刊名称：INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY （ 影响因子：3.713； 五年影响因子：3.408 ）

ISSN： 0950-5423

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收录情况： SCI

摘要： The aim of this study was to investigate the effects of ATP on glycogen phosphorylase activity in lamb during incubation at 4celcius in vitro. Sarcoplasmic proteins from lamb were extracted and treated with different contents of ATP to get three groups of glycogen phosphorylase with low, middle and high ATP content groups, the amount of ATP were 0.5, 2.0 and 2.5 mu M per 100 mu g protein, respectively. The control group was without ATP adding. The results showed that ATP promoted the phosphorylation of glycogen phosphorylase, and phosphorylation modification promoted its activity. But ATP inhibited the activity of glycogen phosphorylase and ATP preferentially participated in phosphorylation. When ATP concentration was 0.5 mu M per 100 mu g protein, the effect of phosphorylation modification on the activity of glycogen phosphorylase was equal to the inhibition of ATP. The effect of glycogen phosphorylase phosphorylation on its activity gradually became dominant as incubation time prolonged.

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