An array of 60,000 antibodies for proteome-scale antibody generation and target discovery

文献类型： 外文期刊

第一作者： Wang, Zhaohui

作者： Wang, Zhaohui;Hou, Bing;Yu, Yuan;Chen, Fulin;Meng, Xun;Li, Yang;Wang, Nan;Chen, Ziqing;Tao, Sheng-ce;Wang, Zhaohui;Hou, Bing;Wang, Mingqiao;Wang, Yuemeng;Cheng, Guangcun;Weng, Weining;Wang, Yiqiang;Tang, Yanfang;Xu, Xuefan;Pan, Rong;Lin, Fei;Meng, Xun;Pronobis, Mira, I;Zheng, Susan;Poss, Kenneth D.;Wang, Shiwei;Ma, Luyan Zulie;Li, Yangrui;Huang, Dongliang;Jiang, Li;Zhang, Qin;Jiang, Li;Zhang, Qin;Wang, Zhiqiang;Zeng, Wenfang;Zhang, Ying;Du, Xuemei;Lin, Ying;Li, Zhiqing;Xia, Qingyou;Geng, Jing;Dai, Huaping;Zhao, Xiao-dong;Tao, Sheng-ce;Yuan, Zheng;Yan, Jian;Yan, Jian;Nie, Qinghua;Zhang, Xiquan;Nie, Qinghua;Zhang, Xiquan;Nie, Qinghua;Zhang, Xiquan;Wang, Kun;Zhu, Yuxian;Wang, Kun;Zhu, Yuxian;Tao, Sheng-ce

作者机构：

期刊名称：SCIENCE ADVANCES （ 影响因子：14.136； 五年影响因子：16.446 ）

ISSN： 2375-2548

年卷期： 2020 年 6 卷 11 期

页码：

收录情况： SCI

摘要： Antibodies are essential for elucidating gene function. However, affordable technology for proteome-scale antibody generation does not exist. To address this, we developed Proteome Epitope Tag Antibody Library (PETAL) and its array. PETAL consists of 62,208 monoclonal antibodies (mAbs) against 15,199 peptides from diverse proteomes. PETAL harbors binders for a great multitude of proteins in nature due to antibody multispecificity, an intrinsic antibody feature. Distinctive combinations of 10,000 to 20,000 mAbs were found to target specific proteomes by array screening. Phenotype-specific mAb-protein pairs were found for maize and zebrafish samples. Immunofluorescence and flow cytometry mAbs for membrane proteins and chromatin immunoprecipitation-sequencing mAbs for transcription factors were identified from respective proteome-binding PETAL mAbs. Differential screening of cell surface proteomes of tumor and normal tissues identified internalizing tumor antigens for antibody-drug conjugates. By finding high-affinity mAbs at a fraction of current time and cost, PETAL enables proteomescale antibody generation and target discovery.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序