Mesorhizobium alexandrii sp. nov., isolated from phycosphere microbiota of PSTs-producing marine dinoflagellate Alexandrium minutum amtk4

文献类型： 外文期刊

第一作者： Yang, Xi

作者： Yang, Xi;Wang, Lei;Yu, Ting;Wang, Zhilin;Bei, Jinlong;Dong, Bo;Dai, Zhangyan;Chen, Zhuang;Jiang, Zhiwei;Zhou, Xin;Zhang, Xiaoling;Yang, Qiao;Zhang, Jing;Zhang, Xiaoling

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关键词： Mesorhizobium alexandrii sp; nov; Alexandrium minutum; Phycosphere microbiota; Paralytic shellfish poisoning toxins; Marine dinoflagellate

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.271； 五年影响因子：2.707 ）

ISSN： 0003-6072

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收录情况： SCI

摘要： An aerobic, Gram-stain-negative, motile and rod-shaped bacterial strain, designated as Z1-4(T), was isolated from the phycosphere microbiota of marine dinoflagellate Alexandrium minutum that produces paralytic shellfish poisoning toxins. Phylogenetic analysis based on 16S rRNA gene sequences showed that the new isolate belongs to the genus Mesorhizobium, and it was closely related to Mesorhizobium waimense LMG 28228(T) and Mesorhizobium amorphae LMG 18977(T) with both 16S rRNA gene sequence similarities of 97.3%. The values of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) relatedness between strain Z1-4(T) and its relatives are both well below the thresholds used for the delineation of a new species. A genome-based phylogenetic tree constructed by up-to-date bacterial core gene set (UBCG) indicates that strain Z1-4(T) forms an independent branch within the genus Mesorhizobium. The respiratory quinone of strain Z1-4(T) was Q-10. The major fatty acids were similar to other members of the genus Mesorhizobium containing the summed feature 8, C-16:0, C-19:0(cyclo)omega 8c, C-17:0 and summed feature 3. The polar lipids are phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, five glycolipids and seven unknown polar lipids. The DNA G + C content was determined to be 62.1 mol % based on its genomic sequence. Combined evidences based on the genotypic, chemotaxonomic and phenotypic characteristics clearly indicates that strain Z1-4(T) represents a novel species of the genus Mesorhizobium, for which the name Mesorhizobium alexandrii sp. nov. is proposed. The type strain is Z1-4(T) (= KCTC 72512(T) = CCTCC AB 2019101(T)).

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