Insights on bio-degumming of kenaf bast based on metagenomic and proteomics

文献类型： 外文期刊

第一作者： Duan, Sheng Wen

作者： Duan, Sheng Wen;Cheng, Li Feng;Feng, Xiang Yuan;Yang, Qi;Liu, Zhi Yuan;Zheng, Ke;Peng, Yuan De

作者机构：

关键词： Bio-degumming; Hibiscus cannabinus; Microbial diversity; iTRAQ

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

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收录情况： SCI

摘要： Background: Microbes play important roles in kanef-degumming. This study aims at identifying the key candidate microbes and proteins responsible for the degumming of kenaf bast (Hibiscus cannabinus). Kenaf bast was cut into pieces and immersed into microbia fermentation liquid collected from different sites. Fermentation liquid samples were collected at 0, 40, 110 and 150 h and then subjected to the 16S/18S rRNA sequencing analysis and isobaric tag for relative and absolute quantitation (iTRAQ) analysis. The microbial (bacterial and fungal) diversity and the differentially expressed proteins/peptides (DEPs) were identified. Results: With the prolonged degumming time, the weight loss rate increased, the bacterial diversity was decreased. [Weeksellaceae], Enterobacteriaceae and Moraxellaceae were rapidly increased at 0 similar to 40 h, and then decreased and were gradually replaced by Bacteroidaceae from 40 h to 150h. Similarly, Chryseobacterium and Dysgonomonas were gradually increased at 0 similar to 110 h and then decreased; Acinetobacter and Lactococcus were increased at 0 similar to 40 h, followed by decrease. Bacteroides was the dominant genus at 150 h. Sequencing 18S rRNA-seq showed the gradually decreased Wallemia hederae and increased Codosiga hollandica during degumming. iTRAQ data analysis showed Rds1, and pyruvate kinase I was decreased and increased in the kanef-degumming, respectively. Other DEPs of ferredoxin I, superoxide dismutase and aconitatehydratase were identified to be related to the Glyoxylate and dicarboxylate metabolism (ko00630). Conclusions: Bacteria including Chryseobacterium, Dysgonomonas, Acinetobacter, Lactococcus and Bacteroidesand fungi like Wallemia hederae and Codosiga hollandica are key candidate microbes for kanef degumming.

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