Characterization of the promoter region of the bovine IRX3 gene: roles of SREBF2 and PPARG

文献类型： 外文期刊

第一作者： Wei, Dawei

作者： Wei, Dawei;Wang, Xingping;Ma, Yun;Zhang, Guijie;Luoreng, Zhuoma;Wei, Dawei;Wang, Xingping;Ma, Yun;Zan, Linsen;Raza, Sayed Haidar Abbas;Khan, Rajwali;Zan, Linsen;Zhang, Jiupan

作者机构：

关键词： IRX3 gene; PPARG; promoter; SREBF2; transcriptional regulation

期刊名称：PHYSIOLOGICAL GENOMICS （ 影响因子：3.107； 五年影响因子：3.446 ）

ISSN： 1094-8341

年卷期： 2020 年 52 卷 3 期

页码：

收录情况： SCI

摘要： As a member of the Iroquois homeobox gene family, the IRX3 gene plays an important role in regulating the growth, development and fat deposition of chordates. In the present study, we found, using real-time PCR, that the bovine IRX3 gene was highly expressed in lung, kidney, heart, subcutaneous fat and longissimus dorsi muscle. We cloned the full-length sequence of the bovine IRX3 gene promoter and constructed eight series of 5' deletion promoter plasmid luciferase reporter assays and then transfected them to 3T3-L1 and C2C12 cell lines to detect its core promoter regions. The results showed that the core promoter of bovine IRX3 was located within a -292/-42 bp region relative to the transcriptional start site. Furthermore, sequence analysis identified eight CpG islands in the promoter region. A chromatin immunoprecipitation assay in combination with site-directed mutation and siRNA interference demonstrated that SREBF2 and PPARG binding occurs in region -292/-42 and is essential in bovine IRX3 transcription. These results lay an important theoretical foundation for exploring the molecular regulation mechanism of the IRX3 gene in bovine fat deposition.

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