Theileria annulata transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells

文献类型： 外文期刊

第一作者： Liu, Junlong

作者： Liu, Junlong;Rashid, Muhammad;Wang, Jinming;Liu, Aihong;Guan, Guiquan;Li, Youquan;Yin, Hong;Luo, Jianxun;Liu, Junlong;Wang, Jinming;Liu, Aihong;Guan, Guiquan;Li, Youquan;Yin, Hong;Luo, Jianxun;He, Lan

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关键词： Theileria annulata; Monocytes; Dendritic cells; Transformation; Phenotypic; Endocytosis

期刊名称：TICKS AND TICK-BORNE DISEASES （ 影响因子：3.744； 五年影响因子：3.693 ）

ISSN： 1877-959X

年卷期： 2020 年 11 卷 3 期

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收录情况： SCI

摘要： Theileria annulata is a protozoan parasite transmitted by ticks to cattle. The most important processes of T. annulata are the infection and transformation of host monocytes, which promote cell division and generate a neoplastic phenotype. Dendritic cells play an important role in the development of adaptive immune responses against parasites and are traditionally classified into four types. One type of dendritic cell derived from afferent lymph was successfully transformed by T. annulata in vitro in a previous report. However, whether the monocyte-derived dendritic cells could be transformed and how the endocytic function is affected by T. annulata infection were not yet known. Bovine dendritic cells (DCs) derived from blood CD14(+) monocytes were co-cultured with T. annulata sporozoites in vitro. On day 15 post infection, rounded and continuously proliferating cells were observed. The effect of this transformation on cell phenotype was studied using immunostaining and flow cytometry. After transformation, the cells maintained the expression of the DC-specific marker CD11c, but it was downregulated as were the expression of CD11b, CD14 and CD86. In contrast, CD205, CD45 and MHC class. molecules were upregulated in transformed cells. The levels of CD172a, CD21, CD40 and CD80 expression were very low in the transformed cells (< 1 %). However, the transformed cells maintained high expression levels of MHC. (> 99 %). In addition, the normal and transformed DCs were cocultured with OVA-FITC antigen to compare the differences of the endocytic functions between these two types of cells. The results revealed that the endocytic functions of MoDCs were significantly inhibited after transformation by T. annulata.

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