Bacillus fungorum sp. nov., a bacterium isolated from spent mushroom substrate

文献类型： 外文期刊

第一作者： Liu, Xin

作者： Liu, Xin;Wang, Lingling;Han, Meilin;Xue, Qian-hui;Gao, Junlian;Sun, Xiaohong;Xue, Qian-hui;Zhang, Gui-shan

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关键词： Bacillus fungorum sp. nov.; spent mushroom substrate; new taxa

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2020 年 70 卷 3 期

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收录情况： SCI

摘要： A facultatively anaerobic, Gram-stain-positive, spore-forming Bacillus strain, 17-SMS-01(T), isolated from spent mushroom substrate in the Fangshan District, Beijing, PR China, was initially identified as a Bacillus cereus group species based on 16S rRNA gene sequences. Strain 17-SMS-01(T) had the highest sequence similarities to Bacillus wiedmannii FSL W8-0169(T) (99.9 %), Bacillus albus N35-10-2(T) (99.9 %), Bacillus luti TD41(T) (99.9 %) and Bacillus proteolyticus TD42(T) (99.9 %). However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain 17-SMS-01(T) and the most closely related species were less than the previously proposed cut-off values of 96% (ANI) and 70% (DDH) for differentiating species within the genus, suggesting that this strain represents a novel Bacillus group species. The fatty acid profile of strain 17-SMS-01(T), which showed a predominance of iso-C-15:0 and anteiso-C-15:0, supported the allocation of the strain to the genus Bacillus. The predominant menaquinone was MK-7 (100%). The major polar lipids were diphosphatidylglycerol, phosphatidyl ethanolamine, phosphatidyl glycerol, an unidentified aminophospholiped and unidentified lipids. The DNA G+C content of the novel strain was 35.0 mol%. The results of physiological and biochemical tests also allowed the phenotypic differentiation of strain 17-SMS-01(T) from the most closely related recognized species. On the basis of the phylogenetic and phenotypic evidence, strain 17-SMS-01(T) represents a novel Bacillus species, for which the name Bacillus fungorum sp. nov. is proposed. Type strain of the novel species is 17-SMS-01(T) (=MCCC 1K03483(T)=KCTC 33949(T)).

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