A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products

文献类型： 外文期刊

第一作者： Zhang, Weiqi

作者： Zhang, Weiqi;Wu, Wenqin;Cai, Chong;Hu, Xiaofeng;Li, Hui;Bai, Yizhen;Zhang, Zhaowei;Li, Peiwu;Hu, Xiaofeng;Li, Hui;Zhang, Zhaowei;Li, Peiwu;Hu, Xiaofeng;Li, Hui;Zhang, Zhaowei;Li, Peiwu;Li, Hui;Zhang, Zhaowei;Li, Hui;Zhang, Zhaowei;Li, Peiwu

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关键词： aflatoxin; mycotoxin; Au@PtNPs; immunoassay; handheld barometer

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN：

年卷期： 2020 年 12 卷 3 期

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收录情况： SCI

摘要： Sensitive and point-of-care detection of small toxic molecules plays a key role in food safety. Aflatoxin, a typical small toxic molecule, can cause serious healthcare and economic issues, thereby promoting the development of sensitive and point-of-care detection. Although ELISA is one of the official detection methods, it cannot fill the gap between sensitivity and point-of-care application because it requires a large-scale microplate reader. To employ portable readers in food safety, Pt-catalysis has attracted increasing attention due to its portability and reliability. In this study, we developed a sensitive point-of-care aflatoxin detection (POCAD) method via a portable handheld barometer. We synthesized and characterized Au@PtNPs and Au@PtNPs conjugated with a second antibody (Au@PtNPs-IgG). A competitive immunoassay was established based on the homemade monoclonal antibody against aflatoxins. Au@PtNPs-IgG was used to catalyze the production of O-2 from H2O2 in a sealed vessel. The pressure of O-2 was then recorded by a handheld barometer. The aflatoxin concentration was inversely proportional to the pressure recorded via the barometer reading. After optimization, a limit of detection of 0.03 ng/mL and a linear range from 0.09 to 16.0 ng/mL were achieved. Recovery was recorded as 83.1%-112.0% along with satisfactory results regarding inner- and inter-assay precision (relative standard deviation, RSD < 6.4%). Little cross-reaction was observed. Additionally, the POCAD was validated by high-performance liquid chromatography (HPLC) by using peanut and corn samples. The portable POCAD exhibits strong potential for applications in the on-site detection of small toxic molecules to ensure food safety.

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