An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera dorsalis Olfaction

文献类型： 外文期刊

第一作者： Liu, Zhao

作者： Liu, Zhao;Liang, Xiao-Fei;Xu, Li;Smagghe, Guy;Wang, Jin-Jun;Keesey, Ian W.;Lei, Zhong-Ren;Smagghe, Guy

作者机构：

关键词： oriental fruit fly; odorant-binding proteins; olfaction; antennae; dsRNA

期刊名称：FRONTIERS IN ECOLOGY AND EVOLUTION （ 影响因子：4.171； 五年影响因子：3.907 ）

ISSN： 2296-701X

年卷期： 2020 年 8 卷

页码：

收录情况： SCI

摘要： Insect antennae are important olfactory organs that house high concentrations of odorant-binding proteins (OBPs) in the sensillum lymph. Previous studies in other insects have shown that OBPs play important roles in transporting odorants and enhancing the sensitivity of the olfactory system. However, the functions of OBPs in the oriental fruit fly, Bactrocera dorsalis, especially those specifically expressed in antennae, have not been fully elucidated. In this study, cDNA libraries were constructed from both the male and female antennal transcriptome, and twenty OBPs were identified in total. The expression profiles of these OBPs were examined in the adult antenna, head, thorax, leg, and abdomen of both sexes. Seven of the identified OBP genes had significantly higher expression in both the male and female antennae than in other tissues, while the transcript levels of the remaining OBPs varied across different tissues. Regarding the function of antenna-specific OBPs, we targeted Bdorsobp2 as a representative for further RNA interference (RNAi) and identified via electrophysiology a decrease in detection of a potential species-specific a potent attractant, methyl eugenol. Moreover, subsequent behavioral assay data showed that the behavioral response of B. dorsalis toward this odorant decreased when Bdorobp2 was silenced with injection of double-stranded RNA (dsRNA). Combined, these results support our initial hypothesis that antennae-specific OBPs are of critical importance for insect odorant detection, sensitivity, and behavior.

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