A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro

文献类型： 外文期刊

第一作者： Xu, Xiaoqing

作者： Xu, Xiaoqing;Peng, Qing;Zhang, Yuwei;Tian, Dandan;Zhang, Pengbo;Huang, Ying;Ma, Lan;Qiao, Yu;Shi, Bo

作者机构：

关键词： Lactobacillus coryniformis NA-3; exopolysaccharide; antioxidant; anti-biofilm; inhibition; dispersion

期刊名称：FOOD & NUTRITION RESEARCH （ 影响因子：3.894； 五年影响因子：4.442 ）

ISSN： 1654-6628

年卷期： 2020 年 64 卷

页码：

收录情况： SCI

摘要： Background: Exopolysaccharides (EPSs) secreted from lactic acid bacteria are carbohydrate polymers with reported biological activities. In this study, we extracted and characterized the composition as well as antioxidant and biofilm-inhibitory properties of EPS from Lactobacillus coryniformis NA-3 isolated from northeast Chinese sauerkraut (Suan Cai). Methods: Lactobacillus coryniformis NA-3 was identified with 16S rDNA amplification and Neighbor Joining (NJ) phylogenetic analysis. EPS derived from Lactobacillus coryniformis NA-3 (EPS-NA3) was analyzed, including compositions by high-performance liquid chromatography (HPI,C), functional groups by Fourier-transform infrared spectroscopy (FT-IR) and glycosidic bond configuration by Hydrogen-1 Nuclear Magnetic Resonance (H-1 NMR). Antioxidant activity of EPS was evaluated with hydroxyl and superoxide radical-scavenging. Anti-biofilm activities of EPS-NA3 were checked through inhibition and dispersion. Results: The monosaccharide composition of EPS included alpha-rhamnose, alpha-mannose, alpha-galactose, and alpha-glucose in a ratio of 2.6:1.0:5.0:3.3. The free radical-scavenging abilities of EPS-NA3 were 37.77% +/- 1.56% and 78.87% +/- 3.07% on hydroxyl and superoxide reactive oxygen species respectively. Moreover, EPS-NA3 attenuated the formation of Bacillus cereus and Salmonella typhimurium biofilms by inhibition ratios of approximately 80% and 40% respectively. Additionally, treatment with EPS-NA3 dispersed established biofilms of B. cereus and S. typhimurium by approximately 90% and 20% respectively. Conclusion: These results suggest that EPS-NA3 may be developed as antioxidant and anti-biofilm agents for industrial and clinical applications due to its capacity of scavenging free radicals, inhibition of bacterial biofilm formation, and dispersion of established biofilms.

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