A Streptomyces sp. NEAU-HV9: Isolation, Identification, and Potential as a Biocontrol Agent against Ralstonia solanacearum of Tomato Plants

文献类型： 外文期刊

第一作者： Ling, Ling

作者： Ling, Ling;Han, Xiaoyang;Li, Xiao;Zhang, Xue;Wang, Han;Zhang, Lida;Cao, Peng;Wu, Yutong;Wang, Xiangjing;Zhao, Junwei;Xiang, Wensheng;Xiang, Wensheng

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关键词： antibacterial activity; Ralstonia solanacearum; Streptomyces sp. NEAU-HV9; actinomycin D

期刊名称：MICROORGANISMS （ 影响因子：4.128； ）

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年卷期： 2020 年 8 卷 3 期

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收录情况： SCI

摘要： Ralstonia solanacearum is an important soil-borne bacterial plant pathogen. In this study, an actinomycete strain named NEAU-HV9 that showed strong antibacterial activity against Ralstonia solanacearum was isolated from soil using an in vitro screening technique. Based on physiological and morphological characteristics and 98.90% of 16S rRNA gene sequence similarity with Streptomyces panaciradicis 1MR-8T, the strain was identified as a member of the genus Streptomyces. Tomato seedling and pot culture experiments showed that after pre-inoculation with the strain NEAU-HV9, the disease occurrence of tomato seedlings was effectively prevented for R. solanacearum. Then, a bioactivity-guided approach was employed to isolate and determine the chemical identity of bioactive constituents with antibacterial activity from strain NEAU-HV9. The structure of the antibacterial metabolite was determined as actinomycin D on the basis of extensive spectroscopic analysis. To our knowledge, this is the first report that actinomycin D has strong antibacterial activity against R. solanacearum with a MIC (minimum inhibitory concentration) of 0.6 mg L-1 (0.48 mu mol L-1). The in vivo antibacterial activity experiment showed that actinomycin D possessed significant preventive efficacy against R. solanacearum in tomato seedlings. Thus, strain NEAU-HV9 could be used as BCA (biological control agent) against R. solanacearum, and actinomycin D might be a promising candidate for a new antibacterial agent against R. solanacearum.

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