Cryptosporidium parvum gp40/15 Is Associated with the Parasitophorous Vacuole Membrane and Is a Potential Vaccine Target

文献类型： 外文期刊

第一作者： Cui, Zhaohui

作者： Cui, Zhaohui;Wang, Luyang;Wang, Yuexin;Wang, Rongjun;Zhang, Longxian;Li, Juan;Sun, Mingfei

作者机构：

关键词： Cryptosporidium parvum; Cpgp40/15; PVM; neutralization; vaccine

期刊名称：MICROORGANISMS （ 影响因子：4.128； ）

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年卷期： 2020 年 8 卷 3 期

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收录情况： SCI

摘要： Cryptosporidium parvum is a zoonotic intracellular protozoan responsible for the diarrheal illness cryptosporidiosis in humans and animals. Although a number of zoite surface proteins are known to be expressed during, and believed to be involved in, attachment and invasion of host cells, the molecular mechanisms by which C. parvum invades the host epithelial cells are not well understood. In the present study, we investigated the gene expression patterns, protein localization in developmental stages in culture, and in vitro neutralization characteristics of Cpgp40/15 and Cpgp40. Indirect immunofluorescence assay showed that Cpgp40/15 is associated with the parasitophorous vacuole membrane (PVM) during intracellular development. Both anti-gp40/15 and anti-gp40 antibodies demonstrated the ability to neutralize C. parvum infection in vitro. Further studies are needed to fully understand the specific role and functional mechanism of Cpgp40/15 (or gp40/15 complex) in the invasion of the host or in the PVM and to determine the feasibility of gp40/15 as a vaccine candidate for cryptosporidiosis in vivo.

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