Targeted Metabolomics Analysis Reveals that Dietary Supranutritional Selenium Regulates Sugar and Acylcarnitine Metabolism Homeostasis in Pig Liver

文献类型： 外文期刊

第一作者： Zhang, Kai

作者： Zhang, Kai;Han, Yunsheng;Zhao, Qingyu;Zhan, Tengfei;Li, Ying;Sun, Wenjuan;Li, Shuang;Sun, Dandan;Si, Xueyang;Yu, Xiaonan;Qin, Yuchang;Tang, Chaohua;Zhang, Junmin;Zhang, Kai;Han, Yunsheng;Zhao, Qingyu;Zhan, Tengfei;Li, Ying;Sun, Wenjuan;Li, Shuang;Sun, Dandan;Si, Xueyang;Yu, Xiaonan;Tang, Chaohua;Zhang, Junmin

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关键词： supranutritional supplementation; SeMet; sugar and acylcarnitine metabolism; liver; pig

期刊名称：JOURNAL OF NUTRITION （ 影响因子：4.798； 五年影响因子：5.718 ）

ISSN： 0022-3166

年卷期： 2020 年 150 卷 4 期

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收录情况： SCI

摘要： Background: The association between high selenium (Se) intake and metabolic disorders such as type 2 diabetes has raised great concern, but the underlying mechanism remains unclear. Objective: Through targeted metabolomics analysis, we examined the liver sugar and acylcarnitine metabolism responses to supranutritional selenomethionine (SeMet) supplementation in pigs. Methods: Thirty-six castrated male pigs (Duroc-Landrace-Yorkshire, 62.0 +/- 3.3 kg) were fed SeMet adequate (Se-A, 0.25 mg Se/kg) or SeMet supranutritional (Se-S, 2.5 mg Se/kg) diets for 60 d. The Se concentration, biochemical, gene expression, enzyme activity, and energy-targeted metabolite profiles were analyzed. Results: The Se-S group had greater fasting serum concentrations of glucose (1.9-fold), insulin (1.4-fold), and free fatty acids (FFAs, 1.3-fold) relative to the Se-A group (P < 0.05). The liver total Se concentration was 4.2-fold that of the Se-A group in the Se-S group (P < 0.05), but expression of most selenoprotein genes and selenoenzyme activity did not differ between the 2 groups. Seven of 27 targeted sugar metabolites and 4 of 21 acylcarnitine metabolites significantly changed in response to high SeMet (P < 0.05). High SeMet supplementation significantly upregulated phosphoenolpyruvate carboxy kinase (PEPCK) activity by 64.4% and decreased hexokinase and succinate dehydrogenase (SDH) activity by 46.5-56.7% (P < 0.05). The relative contents of glucose, dihydroxyacetone phosphate, alpha-ketoglutarate, fumarate, malate, erythrose-4-phosphate, and sedoheptulose-7-phosphate in the Se-S group were 21.1-360% greater than those in the Se-A group (P < 0.05). The expression of fatty acid synthase (FASN) and the relative contents of carnitine, hexanoyl-carnitine, decanoyl-carnitine, and tetradecanoyl-carnitine in the Se-S group were 35-97% higher than those in the Se-A group (P < 0.05). Conclusions: Dietary high SeMet-induced hyperglycemia and hyperinsulinemia were associated with suppression of sugar metabolism and elevation of lipid synthesis in pig livers. Our research provides novel insights into high SeMet intake-induced type 2 diabetes.

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