Phosphoproteomics analysis of hypopharyngeal glands of the newly emerged honey bees (Apis mellifera ligustica)

文献类型： 外文期刊

第一作者： Ahmad, Saboor

作者： Ahmad, Saboor;Li, Jianke;Khan, Khalid Ali;Ghramh, Hamed A.;Khan, Khalid Ali;Ghramh, Hamed A.;Ghramh, Hamed A.

作者机构：

关键词： Newly emerged bees; Hypopharyngeal glands; Mass spectrometry; Phosphoproteomics; Phosphopeptides]

期刊名称：JOURNAL OF KING SAUD UNIVERSITY SCIENCE （ 影响因子：3.829； 五年影响因子：3.973 ）

ISSN： 1018-3647

年卷期： 2022 年 34 卷 6 期

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收录情况： SCI

摘要： The most important honey bee queen food royal jelly is produced by the exocrine hypopharyngeal glands (HGs) of the worker honey bees. The HGs exhibits diverse gene and protein that create age-related physiological adaptations. However, limited knowledge is available on how the phosphorylation process is responsible for physiological alterations across the development of HGs in newly emerged bees. This study measured the acinus of HGs and characterized its phosphoproteomics analysis between the newly emerged bees of royal jelly bees (RJBs) and Italian bees (ITBs). Phosphopeptides of HGs were enriched by Ti4+-IMAC reagents, followed by protein identification via Q-Exactive LC-MS/MS. Our findings indicated that the mean acinus size of HGs of newly emerged bees of RJBs was significantly larger (56.18 +/- 1.72 mu m) than ITBs (45.98 +/- 1.62 mu m). A total of 1576 phosphopeptides with 1800 phosphosites containing 525 phosphoproteins were identified in RJBs, while 746 phosphopeptides, of which 846 phosphosites correspond to 317 phosphoproteins were identified in ITBs. Most proteins were phosphorylated on 1 residue followed by 2 and 3 residues in newly emerged bees of both bee stocks. In addition, serine phosphorylation was most observed, followed by threonine and tyrosine in both bee stocks. In newly emerged bees of RJBs, the protein metabolic process, glycolytic process, and formation of translation preinitiation were uniquely enriched, while protein translation, peptide metabolic process and elongation were enriched in ITBs. This research shows detailed phosphorylation of HGs and provides helpful information for under-standing the biological activities of HGs development in newly emerged bees from both bee stocks. (C) 2022 The Authors. Published by Elsevier B.V. on behalf of King Saud University.

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