RNAi suppression of the nuclear receptor FTZ-F1 impaired ecdysis, pupation, and reproduction in the 28-spotted potato ladybeetle, Henosepilachna vigintioctopunctata

文献类型： 外文期刊

第一作者： Liu, Zhuoqi

作者： Liu, Zhuoqi;Chen, Shimin;Guo, Mujuan;Khan, Muhammad Musa;Qiu, Baoli;Pan, Huipeng;Nanda, Satyabrata;Yang, Chunxiao;Pan, Huipeng;Zhang, Youjun;Zhou, Xuguo

作者机构：

关键词： Henosepilachna vigintioctopunctata; HvFTZ-F1; RNA interference; Ecdysis; Pupation; Reproduction

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.966； 五年影响因子：4.591 ）

ISSN： 0048-3575

年卷期： 2022 年 182 卷

页码：

收录情况： SCI

摘要： Fushi-tarazu factor 1 (FTZ-F1) is an ecdysone-inducible transcription factor that plays a vital role during the metamorphosis in insects. In this study, we functionally characterized HvFTZ-F1 in H. vigintioctopunctata, a dreadful solanaceous crop pest, by using a dietary RNA interference technique. The HvFTZ-F1 expression levels were elevated in the 1st and 2nd-instars before molting and declined immediately after ecdysis. The HvFTZ-F1 silencing led to high mortality in the 1st instars, while the expression of the osmosis-regulative gene, HvAQPAn. G, was significantly increased in the 1st instars. HvFTZ-F1 silencing downregulated the Halloween and 20Erelated genes, decreased the ecdysteroids titer, suppressed the expression of pigmentation-related genes, and reduced the catecholamines titer. In the 4th instars, HvFTZ-F1 silencing caused 100% mortality by arresting the development at the prepupal stage and preventing new abdominal cuticle formation. In the female adults, HvFTZF1 silencing caused an evident decrease in fecundity, prolonged the pre-oviposition period, reduced the number of eggs and hatching rate, severely atrophied the ovaries. Moreover, the 20E-related genes and the dopamine synthesis genes were suppressed in the dsHvFTZ-F1-treated females. Overall, our results revealed that HvFTZ-F1 regulates ecdysis, pupation, and reproduction in H. vigintioctopunctata, thereby could be a promising molecular target for the development of RNAi-based biopesticides to control H. vigintioctopunctata.

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