Odorant binding protein 3 is associated with nitenpyram and sulfoxaflor resistance in Nilaparvata lugens

文献类型： 外文期刊

第一作者： Zhang, Junjie

作者： Zhang, Junjie;Mao, Kaikai;Ren, Zhijie;Jin, Ruoheng;Zhang, Yunhua;Cai, Tingwei;He, Shun;Li, Jianhong;Wan, Hu;Mao, Kaikai

作者机构：

关键词： Nilaparvata lugens; Resistance; Odorant binding protein; Nitenpyram; Sulfoxaflor; Molecular docking

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）

ISSN： 0141-8130

年卷期： 2022 年 209 卷

页码：

收录情况： SCI

摘要： Odorant binding protein (OBP) can interact with small-molecule compounds insecticides and thereby modulate variation in insecticide susceptibility in insects. However, the regulatory mechanism of OBP-mediated insecticide resistance in Nilaparvata lugens, a destructive rice pest in Asia, remains unclear. Here, we explored the role of NlOBP3 in the resistance of N. lugens to nitenpyram and sulfoxaflor. The results showed that NlOBP3 was overexpressed in association with nitenpyram and sulfoxaflor resistance, and NlOBP3 silencing significantly increased the mortality of N. lugens to nitenpyram and sulfoxaflor, suggesting that NlOBP3 may be associated with nitenpyram and sulfoxaflor resistance in N. lugens. OBP localization revealed that NlOBP3 was highly expressed in all nymph stages and was enriched in the antennae, legs, body wall, and fat body. RT-qPCR analyses showed that the mRNA levels of NlOBP3 were significantly affected by nitenpyram and sulfoxaflor. Additionally, molecular docking predicted that there were multiple binding sites that may played key roles in the binding of NlOBP3 with nitenpyram and sulfoxaflor. The current study identifies a previously undescribed mechanism of insecticide resistance in N. lugens, showing that NlOBP3 is likely to be involved in the evolution of nitenpyram and sulfoxaflor resistance in N. lugens.

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