Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes

文献类型： 外文期刊

第一作者： Wu, Xingjie

作者： Wu, Xingjie;Dai, Yuexiu;Peng, Jingjing;Cui, Zhenling;Zhang, Fusuo;Wu, Xingjie;Dai, Yuexiu;Peng, Jingjing;Cui, Zhenling;Zhang, Fusuo;Rensing, Christopher;Han, Dongfei;Xiao, Ke-Qing;Tang, Zhixi;Liesack, Werner

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关键词： phosphorus; gcd; genome; microbiome; metagenomics; MAGs; phosphorous

期刊名称：MSYSTEMS （ 影响因子：7.324； 五年影响因子：9.039 ）

ISSN： 2379-5077

年卷期： 2022 年 7 卷 1 期

页码：

收录情况： SCI

摘要： Enhancing soil phosphate solubilization is a promising strategy for agricultural sustainability, while little is known about the mechanisms of how microorganisms cope with differing phosphorus availability. Using a combination of genome-resolved metagenomics and amplicon sequencing, we investigated the microbial mechanisms involved in phosphorus cycling under three agricultural treatments in a wheat-maize rotation system and two natural reforestation treatments. Available soil phosphorus was the key factor shaping bacterial and fungal community composition and function across our agricultural and reforestation sites. Membrane-bound quinoprotein glucose dehydrogenase (PQQGDH) and exopolyphosphatases (PPX) governed microbial phosphate solubilization in agroecosystems. In contrast, genes encoding glycerol-3-phosphate transporters (ugpB, ugpC, and ugpQ) displayed a significantly greater abundance in the reforestation soils. The gcd gene encoding PQQGDH was found to be the best determinant for bioavailable soil phosphorus. Metagenome-assembled genomes (MAGs) affiliated with Cyclobacteriaceae and Vicinamibacterales were obtained from agricultural soils. Their MAGs harbored not only gcd but also the pit gene encoding low-affinity phos-phate transporters. MAGs obtained from reforestation soils were affiliated with Microtrichales and Burkholderiales. These contain ugp genes but no gcd, and thereby are indicative of a phosphate transporter strategy. Our study demonstrates that knowledge of distinct microbial phosphorus acquisition strategies between agricultural and reforestation soils could help in linking microbial processes with phosphorus cycling. IMPORTANCE The soil microbiome is the key player regulating phosphorus cycling proc-esses. Identifying phosphate-solubilizing bacteria and utilizing them for release of recal-citrant phosphate that is bound to rocks or minerals have implications for improving crop nutrient acquisition and crop productivity. In this study, we combined functional metagenomics and amplicon sequencing to analyze microbial phosphorus cycling proc-esses in natural reforestation and agricultural soils. We found that the phosphorus ac-quisition strategies significantly differed between these two ecosystems. A microbial phosphorus solubilization strategy dominated in the agricultural soils, while a microbial phosphate transporter strategy was observed in the reforestation soils. We further iden-tified microbial taxa that contributed to enhanced phosphate solubilization in the agro-ecosystem. These microbes are predicted to be beneficial for the increase in phosphate bioavailability through agricultural practices.

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