Full-Genome Analysis and Pathogenicity of a Novel Recombinant Field PRRSV-1 Strain Between Amervac-Like and NMEU09-1-Like Strains in China

文献类型： 外文期刊

第一作者： Wei, Chunhua

作者： Wei, Chunhua;Huang, Wenlin;Yang, Yuan;Xie, Feimin;Fan, Kewei;Wang, Hua;Chen, Haoyu;Lan, Xin;Dai, Ailing;Liu, Jiankui;Wei, Chunhua;Xie, Feimin;Fan, Kewei;Dai, Ailing;Liu, Jiankui;Huang, Wenlin;Yang, Yuan;Chen, Haoyu;Luo, Manlin;Zhou, Xia

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关键词： genetic characterization; novel strain; pathogenicity; PRRSV-1; recombination

期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：3.0； 五年影响因子：3.4 ）

ISSN： 1865-1674

年卷期： 2025 年 2025 卷 1 期

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收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) is a major threat to the global swine industry, causing significant economic losses. In recent years, PRRSV-1 has become a growing concern in Chinese pig herds, with an increasing number of clinical infections being reported. However, the pathogenic potential of recombinant PRRSV-1 strains in piglets remains unclear. In this study, a novel recombinant PRRSV-1 strain, PRRSV1/CN/FJFQ-1/2023, was isolated in Fujian Province in 2023, and its pathogenicity for piglets was analyzed. The complete genome of PRRSV1/CN/FJFQ-1/2023 was 15,071 nucleotides (nts) excluding the poly (A) tails, and it had the highest homology (91%) with Amervac PRRS (a live vaccine virus of PRRSV-1). Compared with LV and Amervac vaccine virus, PRRSV1/CN/FJFQ-1/2023 showed distinct genetic features, including a continuous 8-amino acid (aa) deletion in the overlapping region of the ORF3 and ORF4 genes and a unique aa deletion (aa12) in the ORF7 gene. Phylogenetic analysis revealed that PRRSV1/CN/FJFQ-1/2023 belonged to Amervac-like isolates of subtype 1 of PRRSV-1, while recombination analysis showed that it was a recombinant virus from Amervac-like and NMEU09-1-like strains. Animal experiments showed that PRRSV1/CN/FJFQ-1/2023 could induce 100% morbidity, with obvious clinical symptoms, high viremia, and reduced weight gain, without death. In addition, the PRRSV1/CN/FJFQ-1/2023 caused moderate lung consolidation and interstitial pneumonia. Our results suggest that PRRSV1/CN/FJFQ-1/2023 is a moderately pathogenic strain in piglets.

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