Fusion of C3d with hemagglutinin enhances protective immunity against swine influenza virus

文献类型： 外文期刊

第一作者： Li, Guo-Xin

作者： Li, Guo-Xin;Tian, Zhi-Jun;Yu, Hai;Jin, Yuan-Yuan;Hou, Shao-Hua;Zhou, Yan-Jun;Liu, Tian-Qiang;Hu, Shou-Ping;Tong, Guang-Zhi;Li, Guo-Xin;Tong, Guang-Zhi;Li, Guo-Xin;Yu, Hai;Zhou, Yan-Jun;Tong, Guang-Zhi

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关键词： analysis;antibodies;antibody formation;blood serum;brain;DNA;DNA vaccines;effects;ELISA;haemagglutination;immune response;immunity;immunization;influenza;inhibition;kidneys;lesions;neutralization tests;plasmid vectors;viral diseases;virus neutralization;mice;pigs;simian immunodeficiency virus;viruses;China

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

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收录情况： SCI

摘要： H1N1 and H3N2 are the dominant subtypes causing swine influenza in China and other countries. It is important to develop effective vaccines against both H1N1 and H3N2 subtypes of swine influenza virus (SIV). We examined the effects of a DNA vaccine expressing an influenza HA fused to three copies of murine complement C3d in mice. Plasmids encoding soluble HA (sHA), complete HA (tmHA), or a soluble fused form of HA (sHA-mC3d3) were constructed from the H3N2 subtype of SIV. The immune response was monitored by an enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition (HI) assays, and virus neutralization tests. Analysis of antibody titers indicated that immunization with HA-mC3d3 resulted in higher titers of anti-HA antibodies and higher antibody affinities, compared with serum from mice immunized with sHA or tmHA. Furthermore, the C3d fusion increased the Th2-biased immune response, by inducing IL-4 production. Splenocytes from mice immunized with sHA-mC3d3 produced about three-fold more IL-4 than did splenocytes from mice immunized with sHA or tmHA. Seven days post-challenge with homologous virus (H3N2), no virus was isolated from the mice immunized with HA-expressing plasmids. However, 10 days post-challenge with heterologous virus (H1N1), only mice immunized with sHA-mC3d3 had no virus or microscopic lesions in the kidneys and cerebrum. In conclusion, C3d enhanced antibody responses to hemagglutinin and protective immunity against SIV of different subtypes.

分类号： S85

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