High frequency early flowering from in vitro seedlings of Dendrobium nobile

文献类型： 外文期刊

第一作者： Wang, Z. H.

作者： Wang, Z. H.;Wang, L.;Ye, Q. S.;Wang, Z. H.

作者机构：

关键词： Dendrobium nobile Lindl.;In vitro flowering;Seedlings;Temperate orchid

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

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收录情况： SCI

摘要： Dendrobium nobile Lindl. is a popular temperate Chinese orchid commonly marketed as a traditional medicinal plant. Seedlings of Dendrobium nobile Lindl. produced floral buds (33.3-34.8%) precociously on a defined basal medium (1/2 MS) containing paclobutrazol (PP) at 0.5mgLp# or thidiazuron (TDZ) at 0.1mgLp# within 4 months of culturing. The frequency of floral buds formation can be further increased to 95.6% by growing seedlings in a PN (PP 0.3mgLp# +NAA 0.5mgLp#)-containing medium followed by transfer onto 1/2 MS medium with PP and TDZ (PP +TDZ). However, flower developed was deformed under 25pC but it developed fully when grown in a lower temperature regime (23pC/18pC, light/dark) for 45 days. Under optimal condition, in vitro flowering was observed about 6 months after seed sowing.

分类号： S6

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