A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.)

文献类型： 外文期刊

第一作者： Wang, L. H.

作者： Wang, L. H.;Gu, X. H.;Hua, M. Y.;Mao, S. L.;Zhang, Z. H.;Zhang, B. X.;Gu, X. H.;Hua, M. Y.;Yun, X. F.;Peng, D. L.

作者机构：

关键词： Pepper;Capsicum annuum L.;Disease resistance;Root-knot nematode;Meloidogyne spp.;SCAR;N gene;Marker assisted selection

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The root-knot nematodes (Meloidogyne spp.) are important nematode pests and cause serious diseases in pepper in the world. No molecular markers linked to the nematodes resistance N gene have been reported. In this paper, 'Carolina Wonder' (Capsicum annuum L.), a sweet pepper line resistant to root-knot nematode with N gene, '20080-5-29' (C. annuum L.), an inbred line susceptible to root-knot nematode with good horticultural characteristics, and their F progeny with 320 individuals were used as materials. Evaluation of resistance and susceptibility of parental lines, F and F progeny inoculated with root-knot nematodes (Meloidogyne incognita) were carried out. 'Bulked segregant analysis' method was used to search for polymorphic markers from 512 pairs of AFLP primers. Based on the assessment of resistance and susceptibility and polymorphism of the AFLP marker in F population, the genetic linkage distance between the AFLP marker and the N gene was estimated. One AFLP marker E39/M41-339 was obtained and transferred to a SCAR marker amplifying a 315bp DNA fragment linked to the N resistant allele and a 331bp fragment linked to the N susceptible allele. The distance between the molecular marker and the nematodes resistance N gene is 6.3cM. This research delivered a valuable tool for the marker assisted selection of nematodes resistance in pepper.

分类号： S6

  • 相关文献

[1]Bacterial artificial chromosome library construction of root-knot nematode resistant pepper genotype HDA149 and identification of clones linked to Me3 resistant locus. Guo Xiao,Yang Xiao-hui,Yang Yu,Ma Wei-qing,Li Guang-cun,Mao Zhen-chuan,Liu Feng,Xie Bing-yan,Li Guang-cun. 2017

[2]Chinese leek (Allium tuberosum Rottler ex Sprengel) reduced disease symptom caused by root-knot nematode. Huang Yong-hong,Huang Yong-hong,Mao Zhen-chuan,Xie Bing-yan.

[3]Integrated management of root-knot nematodes on tomato in glasshouse production using nematicides and a biocontrol agent, and their effect on soil microbial communities. Liu, Jianbin,Sun, Jingzu,Liu, Xingzhong,Xiang, Meichun,Qiu, Jiyan. 2014

[4]Population diversity of Pasteuria penetrans from pepper fields and its genetic variation from single root-knot nematodes. Zou, XiaoXiao,Xiao, Jun,Huang, HuiQin,Zhang, XiaoLi,Zhang, FuTe,Sun, QianGuang,Liu, Min,Bao, ShiXiang.

[5]Development and application of KASP marker for high throughput detection of AhFAD2 mutation in peanut. Zhao, Shuzhen,Li, Aiqin,Li, Changsheng,Xia, Han,Zhao, Chuanzhi,Zhang, Ye,Hou, Lei,Wang, Xingjun. 2017

[6]Tissue expression and predicted protein structures of the bovine ANGPTL3 and association of novel SNPs with growth and meat quality traits. Chen, N. B.,Ma, Y.,Fu, W. W.,Xu, Y. J.,Li, F.,Yang, T.,Lin, F.,Li, J. Y.,Gao, S. X..

[7]Cloning and Sequence Analysis of N Gene of Transmissible Gastroenteritis Virus HYM-09 Isolated from Dog in China. Yu, Xiaolong,Man, Chaolai. 2012

[8]A Sequence-amplified Characterized Region Marker for a Single, Dominant Gene in Melon PI 134198 that Confers Resistance to a Unique Race of Podosphaera xanthii in China. Liu, Longzhou,Chen, Youyuan,Su, Zhenghong,Zhang, Hui,Zhu, Weiming. 2010

[9]Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus. Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Lei, Zhi-Hai,Zeng, Jiang-Yong,Zhu, Se,Duoji, Ciren,Dunzhu, Luobu. 2013

[10]Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.). Xu Liang,Jiang Qiu-wei,Wang Yan,Gong Yi-qin,Wang Xian-li,Limera Cecilia,Liu Li-wang,Wu Jian. 2014

[11]Development and application of a molecular marker for TMV resistance based on N gene in tobacco (Nicotiana tabacum). Zhang Yu,Wen Liuying,Yang Aiguo,Luo Chenggang,Cheng Lirui,Jiang Caihong,Chang Aixia,Wang Yuanying,Li Wei,Zhang Jing,Xiao Zhixin.

[12]New mutations existing in the nucleocapsid (N) gene of non-porcine TGEV strains isolated in China. Yu, Xiaolong,Man, Chaolai. 2011

[13]Identification of a three-base deletion in the Pi2 locus, and development of functional marker for marker-assisted resistance selection. Sheng, Zhonghua,Wei, Xiangjin,Xie, Lihong,Jiao, Guiai,Shao, Gaoneng,Tang, Shaoqing,Hu, Peisong,Li, Qianlong,Li, Wei,Chen, Wei,Wang, Jianlong.

[14]Confirmation and Sequence analysis of N gene of PPRV in South Xinjiang, China. Liu, YongHong,Li, XianQiang,Zhang, Luyao,Li, Fei,Zhao, Li,Yang, Bo,Wang, XueFeng. 2017

[15]Integration of marker-assisted selection for resistance to pre-harvest sprouting with selection for grain-filling rate in breeding of white-kernelled wheat for the Chinese environment. Xiao, Shi-He,You, Guang-Xia,Zhang, Xiu-Ying,Yan, Chang-Sheng,Chen, Xu,Zhang, Hai-Ping. 2012

[16]Rapid differentiation of vaccine strain and Chinese field strains of transmissible gastroenteritis virus by restriction fragment length polymorphism of the N gene. Wang, Chengbao,Chen, Jianfei,Shi, Hongyan,Qiu, Hua-Ji,Xue, Fei,Liu, Shengwang,Liu, Changming,Zhu, Yuanmao,Feng, Li,Almazan, Fernando,Enjuanes, Luis. 2010

[17]Characterization and molecular mapping of RsrR, a resistant gene to maize head smut. Li, W. H.,Guo, L. Q.,Wu, S. W.,Weng, M. L.,Jin, D. M.,Wang, B.,Li, W. H.,Wu, J. Y.,Xu, X. D.,Jiang, Y.,Dong, H. Y.,Li, G.,Ru, Z. G..

[18]A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens. Zhu, Yu,Wang, Chunren,Cui, Yudong,Cui, Shangjin,Zhu, Yu,Liang, Lin,Luo, Yakun,Cui, Shangjin,Wang, Guihua,Ai, Xia.

[19]Evaluation of the combination of 1,3-dichloropropene and dazomet as an efficient alternative to methyl bromide for cucumber production in China. Mao, Lian-Gang,Wang, Qiu-Xia,Yan, Dong-Dong,Xie, Hong-Wei,Li, Yuan,Guo, Mei-Xia,Cao, Ao-Cheng.

[20]Nematode communities in continuous tomato-cropping field soil infested by root-knot nematodes. Shi, L. B.,Wu, H. Y.,Zheng, G. D.,Peng, D. L.. 2012

作者其他论文 更多>>

微信小程序