Long-term cryopreservation of sperm from Mandarin fish Siniperca chuatsi

文献类型： 外文期刊

第一作者： Ding, Shuyan

作者： Ding, Shuyan;Ge, Jiachun;Hao, Chen;Zhang, Mingsheng;Yan, Weihui;Xu, Zhiqiang;Pan, Jianlin;Chen, Songlin;Tian, Yongsheng;Huang, Yahong

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关键词： chilling;cryopreservation;effects;eggs;fertilization;freezing;hatching;methodology;semen;sexual reproduction;spermatozoa;swimming;techniques

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

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收录情况： SCI

摘要： In order to develop cryopreservation techniques for long-term preserving the sperm of Mandarin fish Siniperca chuatsi, we examined the effects of various extender and cryopreservation on post-thaw motility. We found the optimal freezing procedures for the Mandarin fish sperm is diluting the semen in D-15 extender, chilling it to 4pC, adding MESO to a final concentration of 10% (v/v), then transferring the semen in cryotubes, holding the cryotubes for 10min at 6cm (about -180pC) above the surface of liquid nitrogen, for 5min on the surface of liquid nitrogen, and finally plunged into liquid nitrogen. After thawed at 37pC for 60s, the sperm had the highest post-thaw motility (96.00pl1.73%). The optimal fertilization procedures for the frozen sperm is mixing the eggs with sperm, then adding 1ml of swimming medium (SM=45mM NaCl+5mM KCl+20mM Tris-HCl, pH 8.0) immediately. At the sperm/egg ratio of 100,000:1, the fertilization rate and the hatching rate of the frozen sperm cryopreserved for 1 week or 1 year in liquid nitrogen (66.01pl5.14% and 54.76pl4.40% & 62.97pl14.28% and 52.58pl11.17%) were similar to that of fresh sperm (69.42pl8.11% and 59.82pl5.27%) (p >0.05). This is the first report that the Mandarin fish (S. chuatsi) sperm can successfully fertilized eggs after long-term cryopreservation.

分类号： Q95

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