Phytase production by fermentation of recombinant Pichia pastoris in monosodium glutamate wastewater

文献类型： 外文期刊

第一作者： Bai, Yingguo

作者： Bai, Yingguo;Yang, Peilong;Wang, Yaru;Shi, Pengjun;Luo, Huiying;Meng, Kun;Wu, Bo;Yao, Bin

作者机构：

关键词： Monosodium glutamate wastewater (MSGW);Phytase;Pichia pastoris;Response surface methodology (RSM);Single-cell protein (SCP)

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A novel method is proposed to produce both phytase and single-cell protein in recombinant Pichia pastoris fermentation using monosodium glutamate wastewater (MSGW) as the basal medium. Recombinant P. pastoris MR33 transformed with a phytase gene (AppA-m) from Escherichia coli was constructed and showed capability to utilize ammonium as the only nitrogen source. The fermentation medium was optimized in shake flasks by single-factor test and response surface methodology. A fed-batch system containing 30% MSGW, 50 g/l glucose, 1.58 g/l CaSO, 5.18 g/l MgSO and 6.67 g/l KHPO was developed in a 3.7-l bioreactor. The maximum phytase activity in the MSGW medium reached 3,380 U/ml, 84.2% of that in chemically defined medium, and the dry cell weight was 136 g/l. The single-cell protein (SCP; 46.66% dry cell weight) contains a variety of amino acids and is low in fat, which is ideal for utilization in animal feed. Thus, it is feasible to use MSGW medium for the production of enzymes that can be expressed in P. pastoris.

分类号： Q93

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