Effect of Vitrification on Mitochondrial Distribution and Membrane Potential in Mouse Two Pronuclear (2-PN) Embryos

文献类型： 外文期刊

第一作者： Zhao, Xue-Ming

作者： Zhao, Xue-Ming;Fu, Xiang-Wei;Hou, Yun-Peng;Yan, Chang-Liang;Suo, Lun;Wang, Yan-Ping;Zhu, Shi-En;Zhao, Xue-Ming;Fu, Xiang-Wei;Hou, Yun-Peng;Yan, Chang-Liang;Suo, Lun;Wang, Yan-Ping;Zhu, Shi-En;Zhao, Xue-Ming;Zhu, Hua-Bin;Dinnyes, Andras

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关键词： cell membrane potential;microtubule distribution;mitochondrial distribution;embryo developmental ability;mitochondrial ring formation

期刊名称：MOLECULAR REPRODUCTION AND DEVELOPMENT （ 影响因子：2.609； 五年影响因子：3.47 ）

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收录情况： SCI

摘要： The present study was designed to investigate the effect of vitrification on mitochondrial distribution, membrane potential (Ay) and microtubule distribution in mouse 2-PN embryos, as well as to document the relationship between mitochondrial distribution and developmental ability of those embryos. Mitochondrial distribution was examined by fluorescence microscopy technology. Results indicated that: (1) The rate of mitochondrial ring formation around pronuclei in vitrified 2-PN embryos was significantly lower than in fresh ones (67.3 +/- 3.0% vs. 84.9 +/- 3.1%) (P < 0.05). (2) Blastocyst development rate of vitrified 2-PN embryos without mitochondrial rings (61.7 +/- 4.5%) was significantly lower than that of vitrified embryos with mitochondrial rings (82.1 +/- 2.8%). (3) Following staining by 5,5',6,6'-tetrachloro-1, 1',3,3'-tetraethyl-imidacarbo-cyanine iodide (JC-1), most red-colored mitochondria (high Delta psi) were distributed peripherally around pronuclei and along cell membranes of fresh 2-PN embryos. Conversely, red-colored mitochondria were greatly diminished in vitrified embryos, with green mitochondria (low Delta psi) evenly distributed throughout the cytoplasm. The proportion of fresh 2-PN embryos with obvious aggregation of high Ay mitochondria (84.2 +/- 2.2%) was significantly higher than that of vitrified embryos (26.7 +/- 3.0%) (P < 0.05). (4) The proportion of fresh embryos with micro-tubules distributed around pronuclei (83.5 +/- 3.4%) was similar to that of vitrified embryos (74.7 +/- 2.5%). In conclusion, vitrification affected mitochondrial distribution and decreased the mitochondrial membrane potential in mouse 2-PN embryos, events which may affect subsequent developmental viability of such embryos. Mol. Reprod. Dev. 76: 1056-1063, 2009.

分类号： Q7

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