Alteration of Fecal Microbiota, Fecal Metabolites, and Serum Metabolites in Dairy Cows with Pre-Retained Placenta

文献类型： 外文期刊

第一作者： Zhou, Tao

作者： Zhou, Tao;Du, Zhenlong;Luo, Zhengzhong;Wu, Dan;Huang, Yixin;Yao, Xueping;Shen, Liuhong;Yu, Shumin;Cao, Suizhong;Zhou, Tao;Du, Zhenlong;Yan, Zuoting;Li, Xiaoping;Yong, Kang

作者机构：

关键词： dairy cows; retained placenta; early-warning markers; omics technology

期刊名称：METABOLITES （ 影响因子：3.4； 五年影响因子：4.0 ）

ISSN：

年卷期： 2024 年 14 卷 7 期

页码：

收录情况： SCI

摘要： Retained placenta (RP) affects lactation and fertility in dairy cows and causes economic losses to the dairy industry. Therefore, screening for early warning of this disease is important. This study used multi omics techniques to reveal the metabolic differences of dairy cows before RP onset and to find potential warning markers. Fecal samples and serum samples of 90 healthy Holstein cows were collected 7 days pre-calving; 10 healthy and 10 RP cows were enrolled according to normal expulsion of fetal membranes after calving. Fecal samples were subjected to 16S rRNA sequencing and untargeted metabolomics analysis, while plasma was analyzed using targeted metabolomics. Pathogenic bacteria levels increased in the intestines of cows with RP compared to those in healthy cows. Lipid metabolites constituted the largest proportion of differential metabolites between feces and plasma. Six potential warning markers for RP in cows were identified, including two fecal microbiomics markers (Oscillospiraceae UCG-005 and Escherichia-Shigella), one fecal untargeted metabolomics marker (N-acetylmuramic acid), and three plasma targeted metabolomics markers (glycylcholic acid-3 sulfate, 7-ketolithocholic acid, and 12-ketolithocholic acid). These biomarkers can predict RP occurrence in the early perinatal period. These results lay a theoretical foundation for early nutritional intervention and pathogenesis research in dairy cows.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序