Construction and immunogenicity analysis of a recombinant baculovirus targeting the N protein of SARS-CoV-2

文献类型： 外文期刊

第一作者： Yu, Bohan

作者： Yu, Bohan;Mo, Xiaoli;Sui, Li;Fang, Yujia;Tang, Xudong;Qian, Ping;Tang, Xudong;Qian, Ping

作者机构：

关键词： Baculovirus; COVID-19 N protein; Immunogenicity protein expression; Sodium butyrate

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.1； 五年影响因子：2.5 ）

ISSN： 0141-5492

年卷期： 2025 年 47 卷 3 期

页码：

收录情况： SCI

摘要： ObjectivesThis study aimed to construct recombinant baculoviruses capable of expressing the nucleocapsid N protein of SARS-CoV-2 and to assess the effects of co-administration with sodium butyrate on its expression and immunogenicity.ResultsThe recombinant BmNPV expressed green fluorescent protein in BmN cells and N protein in mammalian cells. The addition of sodium butyrate significantly enhanced the expression of N protein in HEK293T cells. Following intramuscular injection of recombinant BmNPV into mice, specific antibodies against the N protein were detectedon day 7, 21, and 35. Co-administration with sodium butyrate (1000 mg/kg body weight) significantly enhanced the immunogenicity of the recombinant virus.ConclusionsRecombinant BmNPV expressing the SARS-CoV2 N protein successfully inducedthe anti-N immunogenicity in mice, providing a solid foundationfor the development of novel subunit vaccines against SARS-CoV-2.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序