Development of a real-time recombinase-aided amplification (RT-RAA) molecular diagnosis assay for sensitive and rapid detection of Toxoplasma gondii

文献类型： 外文期刊

第一作者： Wang, Zhao-Hua

作者： Wang, Zhao-Hua;Zhang, Xiu-Zhong;Yao, Xin-Ran;Huang, Wei;Wang, Xiao-Jia;Zhang, Wei;Jia, Hong;Hou, Shao-Hua;Liu, Xiao-Lei

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关键词： Real-time recombinase-aided amplification; Toxoplasma gondii; Rapid detection

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

ISSN： 0304-4017

年卷期： 2021 年 298 卷

页码：

收录情况： SCI

摘要： Toxoplasma gondii, a protozoan intracellular parasite, is present in a wide range of hosts, including virtually all species of warm-blooded vertebrates. Toxoplasmosis spreads to humans through a variety of pathways, including contaminated food or water, and close contact with various types of domestic animals. It poses a severe threat to human health, and contributes to important economic losses, not only in cost-of-illness but also in surveillance programs. It is thus necessary to develop a rapid point-of-care field diagnostic technology to control or prevent pathogen transmission to economically important livestock animals, domestic animals, and human beings. In this study, we develop a real-time isothermal amplification method capable of detecting the T. gondii genome in swine and feline blood samples. This method can detect toxoplasma genome with a lowest detection limit of 10(2) copies of per reaction under optimal reaction conditions of 36 degrees C for 25 min. The assay displayed advantages in sensitivity and specificity in comparison to traditional real-time PCR, and can be performed in a portable instrument.

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