Efficient marker-assisted breeding for clubroot resistance in elite Pol-CMS rapeseed varieties by updating the PbBa8.1 locus

文献类型： 外文期刊

第一作者： Guo, Yiming

作者： Guo, Yiming;Li, Bao;Li, Mei;Yang, Qian;Liu, Xinhong;Qu, Liang;Fan, Lianyi;Wang, Tonghua;Guo, Yiming;Li, Bao;Li, Mei;Yang, Qian;Liu, Xinhong;Qu, Liang;Fan, Lianyi;Wang, Tonghua;Zhu, Hongjian

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关键词： Brassica napus; Clubroot resistance; Backcross breeding; Marker-assisted selection; KASP platform

期刊名称：MOLECULAR BREEDING （ 影响因子：3.297； 五年影响因子：3.011 ）

ISSN： 1380-3743

年卷期： 2022 年 42 卷 7 期

页码：

收录情况： SCI

摘要： Clubroot disease poses a severe threat to rapeseed (Brassica napus) production worldwide and has recently been spreading across China at an unprecedented pace. Breeding and cultivation of resistant varieties constitute a promising and environment-friendly approach to mitigating this threat. In this study, the clubroot resistance locus PbBa8.1 was successfully transferred into SC4, a shared paternal line of three elite varieties in five generations by marker-assisted backcross breeding. Kompetitive allele specific PCR (KASP) markers of clubroot resistance gene PbBa8.1 and its linked high erucic acid gene (FAE1) were designed and applied for foreground selection, and 1,000 single-nucleotide polymorphisms (SNPs) were selected and used for the background selection. This breeding strategy produced recombinants with the highest recovery ratio of the recurrent parent genome (> 95%) at BC2F2 while breaking the linkage with FAE1 during the selection. An updated version of the paternal line (SC4R) was generated at BC2F3, showing significantly improved clubroot resistance at the seedling stage via artificial inoculation, and was comparable to that of the donor parent. Field trials of the three elite varieties and their updated versions in five environments indicated similar agronomic appearance and final yield. The introduced breeding strategy precisely pyramids the PbBa8.1 and FAE1 loci with the assistance of technical markers in a shorter period and could be applied to other desirable traits for directional improvement in the future.

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