Purification and expression of a protein elicitor from Alternaria tenuissima and elicitor-mediated defence responses in tobacco

文献类型： 外文期刊

第一作者： Liu, Q.

作者： Liu, Q.;Yang, X.;Long, C.;Zhao, M.;Zeng, H.;Liu, H.;Yuan, J.;Qiu, D.

作者机构：

关键词： Alternaria tenuissima;protein elicitor;SAR;TMV;tobacco

期刊名称：ANNALS OF APPLIED BIOLOGY （ 影响因子：2.75； 五年影响因子：2.942 ）

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年卷期：

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收录情况： SCI

摘要： A new protein elicitor, PeaT1, was purified from the mycelium of Alternaria tenuissima by column chromatography. PeaT1 was identified as a heat-stable and acidic protein. It induced systemic acquired resistance to tobacco mosaic virus (TMV) in tobacco plants but did not cause hypersensitive response. The elicitor-encoding gene was cloned by rapid amplification of cDNA ends method. Sequence analysis revealed that the cDNA is 624 bp in length and the open reading frame encodes for a polypeptide of 207 amino acids with a nascent polypeptide-associated complex domain. The peaT1 gene was cloned into the expression vector pET-28a and transformed into Escherichia coli BL21 (DE3). The recombinant elicitor also triggered defence responses in intact tobacco plants. The availability of the pure protein offers the possibility to isolate the corresponding receptor and links it to the downstream signalling pathway.

分类号： Q1-0

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