Microbial community analysis in crab ponds by denaturing gradient gel electrophoresis

文献类型： 外文期刊

第一作者： Cheng, Yan Fen

作者： Cheng, Yan Fen;Zhou, Qun Lan;Xie, Jun;Ge, Xian Ping;Liu, Bo;Zhu, Wei-Yun

作者机构：

关键词： water quality;microbial community;seasonal effect;biogeochemical cycling;aeration effect;aquatic ecology

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： In order to investigate the role of microbial community in aquatic ecology and biogeochemical cycles, the bacterial community in crab ponds was investigated and the effects of aeration and season on the bacterial community were also assessed. Total DNAs from the water samples were amplified with universal primers and the amplicons were then resolved by denaturing gradient gel electrophoresis. Bands from the resulting profiles were excised and sequenced. Cluster analysis of the resulting profiles showed that the microbial community was affected by aeration and season. The microbial community between the surface and bottom of the water was very similar. A total of fifteen bands were obtained in this study. Three of them were 91-99% similar to uncultured bacterium clones. Three were 95-99% similar to uncultured Verrucomicrobia bacteria. Three were 97-100% similar to Actinobacterium sp.. Two were similar to Candidatus Limnoluna rubra with similarity 96 and 99%, respectively. Four were 99% similar to Rhodococcus sp., 100% similar to Sporosarcina sp., 100% similar to Stenotrophomonas sp., and 98% similar to Hydrogenophaga sp., respectively. The concentrations of dissolved oxygen, total nitrogen, total phosphorous, nitrite, and ammonia and pH values were significantly affected by season while only the pH value and the concentrations of dissolved oxygen and total nitrogen were significantly affected by aeration.

分类号： Q93

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