Establishment of a high-sensitivity indirect competitive ELISA for florfenicol in chicken meat and egg
文献类型： 外文期刊
第一作者： Ge, M.
作者： Ge, M.;Xing, Y.;Sun, Y.;Cai, W.;Hu, X.;Ge, M.;Cai, W.;Wang, Y.
作者机构：
关键词： florfenicol; high sensitivity; ic-ELISA; chicken meat; egg
期刊名称：ACTA ALIMENTARIA （ 影响因子：1.0； 五年影响因子：1.1 ）
ISSN： 0139-3006
年卷期： 2025 年 54 卷 2 期
页码：
收录情况： SCI
摘要： The aim was to develop a highly sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method for detecting florfenicol (FF) in chicken meat and eggs. The concentrations of the coated antigen and monoclonal antibodies of FF and the reaction conditions were optimised. The performance of the established method was thoroughly evaluated. The results indicated that the detection limit (LOD) was 0.011 mu g kg-1 with an IC50 of 0.224 mu g kg-1 and a detection range of 0.011-4.538 mu g kg-1. The coefficient of variation between and within batches was less than 10%. The spiked recoveries of chicken meat samples ranged from 90.10 to 95.55%, while the spiked recoveries of egg samples ranged from 91.28 to 97.56%. In conclusion, the developed ic-ELISA method is highly sensitive and suitable for monitoring and detecting trace FF residues in chicken meat samples.
分类号：
- 相关文献
作者其他论文 更多>>
-
Metabolite analysis of peach (Prunus persica L. Batsch) branches in response to freezing stress
作者：Li, Y.;Wang, Y.;Wang, Z.;Liu, G.;Chang, R.;Chen, H.;Li, J.;Tian, Q.
关键词：Freezing stress; Metabolic networks; Metabolism; Peach
-
Effects of Biochar and α-Fe2O3 Nanoparticles on the Growth, Photosynthetic Apparatus and Fruit Quality of Medicinal Plant Muskmelon
作者：Wang, Y.;Zhou, W.;Yi, L.;Dai, Z.;Qiu, L.;Zhou, Y.;Wang, S.;Li, H.;Li, J.;Wang, Y.;Zhou, W.;Yi, L.;Dai, Z.
关键词：Cucumis melo; alpha-Fe2O3 nanoparticles; biochar; medicinal ingredients; muskmelon; fruit quality
-
Cytosine base editor-mediated high-efficiency myostatin editing in Hu sheep
作者：Wang, Y.;Liu, W. J.;Meng, C. H.;Wang, H. L.;Cui, Z. K.;Zhang, J.;Zhang, J. L.;Qian, Y.;Li, Y. X.;Cao, S. X.;Wang, Y.;Liu, W. J.;Meng, C. H.;Wang, H. L.;Cui, Z. K.;Zhang, J.;Zhang, J. L.;Qian, Y.;Li, Y. X.;Cao, S. X.;Wang, Y.;Liu, W. J.;Meng, C. H.;Wang, H. L.;Cui, Z. K.;Zhang, J.;Zhang, J. L.;Qian, Y.;Li, Y. X.;Cao, S. X.
关键词：Hu sheep; MSTN; CBE; Off-target effects
-
Isolation and Degradation Characteristics of a Novel Halophilic Polycyclic Aromatic Hydrocarbon-Degrading Bacterium
作者：Cao, H.;Wang, S.;Guo, L.;Zhao, B.;Wang, H.;Wang, Y.;Cao, H.
关键词：bioremediation; halophilic bacterium; PAH biodegradation; degradation pathway;
Stutzerimonas frequens -
Molecular and transcriptomic analysis of the ovary during laying and brooding stages in Zhedong white geese (Anser cygnoides domesticus)
作者：Wang, Y.;Wang, S.;Zang, Z.;Li, B.;Huang, H.;Liu, G.;Zhao, X.
关键词：Reproductive hormones; follicles; apoptosis; mTOR, geese; ovary
-
Study on the diversity of rust pathogens from different hosts in Guizhou Province, China
作者：Sun, J. E.;Chen, X. J.;Wang, Y.;Yang, Z. F.;Liu, F. Q.;Fu, L.;Norphanphoun, C.;Hyde, K. D.;Norphanphoun, C.;Hyde, K. D.;Chen, X. J.;Yu, L. F.;Mckenzie, E. H. C.
关键词：Host jumping; Molecular phylogeny; Plant disease; Rust fungi; Taxonomy
-
Polyphasic taxonomy clarifies the relationships between Butyriboletus and Exsudoporus , and new taxa and reports of Boletaceae from China
作者：Wang, Y.;Yang, Xy;Liu, Yj;Rao, G.;Gui, Xy;Wang, L. Y.;Zhang, B.;Li, Y.;Wang, Y.;Li, Y.;Ma, Yh;Wu, G.;Wu, G.;Dai, D.;Chen, X.
关键词：16 new taxa; basidiomycota; biogeography; evolution; long-distance dispersal; phylogeny
