Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides)
文献类型: 外文期刊
第一作者: Shi, Yan
作者: Shi, Yan;Zhu, Xin-Ping;Yin, Jing-Kui;Zhang, Qi-Ya;Gui, Jian-Fang;Zhu, Xin-Ping
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关键词: apoptosis;immune response;cell growth regulation;antiviral defense
期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )
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收录情况: SCI
摘要: Interferon-regulatory factor 1 (IRF-1) is the first member of IRF family, which is involved in many biological processes such as immune response, antiviral defense, cell growth regulation, and apoptosis. In this study, an IRF-1 gene, EcIRF-1, was isolated and characterized from orange-spotted grouper (Epinephelus coioides). The full-length cDNA of EcIRF-1 is 1,730 bp, including an open reading frame of 906 bp, a 5o-terminal untranslated region (5o-UTR) of 153 bp, and a 3o-UTR of 671 bp. The EcIRF-1 gene consists of 10 exons and 9 introns, spanning over approximate 4.3 kb of genomic sequence. The 5o-UTR sequence contains an exon and an intron, and the 3o-UTR sequence is included in the last exon. Expression analysis by real-time PCR reveals that the EcIRF-1 gene is ubiquitously expressed in various healthy fish tissues, whereas its expression is upregulated in vivo in response to polyinosinic-polycytidylic acid or lipopolysaccharide stimulation. Subcellular localization analysis shows the EcIRF-1 is an intranuclearly localized and immobile protein in the cultured fish cells. Data presented in this paper provide an important base to further understand EcIRF-1 gene function and its regulation associated with interferon immune system in orange-spotted grouper.
分类号: Q7
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