Isopentenyl transferase gene (ipt) downstream transcriptionally fused with gene expression improves the growth of transgenic plants

文献类型： 外文期刊

第一作者： Guo, Jian-Chun

作者： Guo, Jian-Chun;Duan, Rui-Jun;Fu, Shao-Ping;Hu, Xin-Wen;Li, Kai-Mian

作者机构：

关键词： Isopentenyl transferase;Transcriptional fusion;Plant growth and development;Transgenic plants

期刊名称：TRANSGENIC RESEARCH （ 影响因子：2.788； 五年影响因子：2.377 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： This research reports a promising approach to increase a plant's physiological cytokinin content. This approach also enables the increase to play a role in plant growth and development by introducing the ipt gene to downstream transcriptionally fuse with other genes under the control of a CaMV35S promoter, in which the ipt gene is far from the 35S promoter. According to Kozak's ribosome screening model, expression of the ipt gene is reduced by the terminal codon of the first gene and the internal untranslated nucleotides between the fused genes. In the transgenic plants pVKH35S-GUS-ipt, pVKH35S-AOC-ipt, and pVKH35S-AtGolS2-ipt, cytokinins were increased only two to threefold, and the plants grew more vigorously than the pVKH35S-AOC or pVKH35S-AtGolS2 transgenic plants lacking the ipt gene. The vigorous growth was reflected in rapid plant growth, a longer flowering period, a greater number of flowers, more seed product, and increased chlorophyll synthesis. The AOC and AtGolS2 genes play a role in a plant's tolerance of salt or cold, respectively. When the ipt gene transcriptionally fuses with AOC or AtGolS2 in the frame of AOC-ipt and AtGolS2-ipt, slight cytokinin increases were obtained in their transgenic plants; furthermore, those increases played a positive role in improvements of plant growth. Notably, an increased cytokinin volume at the physiological level, in concert with AtGolS2 expression, enhances a plant's tolerance to cold.

分类号： R394

  • 相关文献

[1]Improved salt tolerance and delayed leaf senescence in transgenic cotton expressing the Agrobacterium IPT gene. Y.D. Liu,Z.J. Yin,J.W. YU,J. LI,H.L. WEI,X.L. HAN,F.F. SHEN.

[2]Functional Characterization of the Versatile MYB Gene Family Uncovered Their Important Roles in Plant Development and Responses to Drought and Waterlogging in Sesame. Mmadi, Marie Ali,Dossa, Komivi,Wang, Linhai,Zhou, Rong,Wang, Yanyan,Zhang, Xiurong,Mmadi, Marie Ali,Dossa, Komivi,Cisse, Ndiaga,Mmadi, Marie Ali,Dossa, Komivi,Sy, Mame Oureye. 2017

[3]AtRabD2b, a Functional Ortholog of the Yeast Ypt1, Controls Various Growth and Developmental Processes in Arabidopsis. Wang, Fang,Liu, Chao,Wei, Chen,Zheng, Qian,Wu, Jiangsheng,Liu, Kede,Wang, Fang,Liu, Chao,Wei, Chen,Zheng, Qian,Wu, Jiangsheng,Liu, Kede,Cui, Yuhai,Zhang, Jiaming.

[4]Analysis of differentially expressed genes in response to endogenous cytokinins during cotton leaf senescence. P. ZHAO,N. ZHANG,Z.J. YIN,Y.D. LIU,F.F. SHEN. 2013

[5]Two tobacco AP1-like gene promoters drive highly specific, tightly regulated and unique expression patterns during floral transition, initiation and development. Zhang, Jinjin,Yan, Guohua,Wen, Zhifeng,Liu, Zongrang,Zhang, Jinjin,Yan, Guohua,Wen, Zhifeng,An, Young-Qiang,Singer, Stacy D.. 2014

[6]Expression of kenaf mitochondrial chimeric genes HM184 causes male sterility in transgenic tobacco plants. Liao, Xiaofang,Huang, Zhipeng,Chen, Peng,Zhou, Bujin,Liu, Dongmei,Kong, Xiangjun,Zhou, Ruiyang,Zhao, Yanhong. 2015

[7]Expression of the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1 in peanut to improve drought and salt tolerance. Qin, Hua,Zhang, Yizheng,Gu, Qiang,Kuppu, Sundaram,Sun, Li,Zhu, Xunlu,Mishra, Neelam,Hu, Rongbin,Zhang, Hong,Shen, Guoxin,Zhang, Junling,Burow, Mark,Zhu, Longfu,Zhang, Xianlong,Payton, Paxton. 2013

[8]Evaluation of transgenic Bt corn for resistance to the Asian corn borer (Lepidoptera : Pyralidae). Wang, ZY,Zhou, DR,Wen, LP,Song, YY,Yao, ZY. 2003

[9]Brazil and the development of international scientific biosafety testing guidelines for transgenic crops. Capalbo, DMF,Hilbeck, A,Andow, D,Snow, A,Bong, BB,Wan, FH,Fontes, EMG,Osir, EO,Fitt, GP,Johnston, J,Songa, J,Heong, KL,Birch, ANE. 2003

[10]Transgenic expression of a rice riboflavin synthase gene in tobacco enhances plant growth and resistance to Tobacco mosaic virus. Zhao, Y. Y.,Wang, D. F.,Dong, H. S.,Zhang, C. L.,Wu, T. Q.,Guo, A.. 2014

[11]Isolation and identification of a wheat gene encoding a zinc finger protein (TaZnFP) responsive to abiotic stresses. Min, Dong-Hong,Zhao, Yue,Huo, Dong-Ying,Li, Lian-Cheng,Chen, Ming,Xu, Zhao-Shi,Ma, You-Zhi.

[12]Co-ordinate expression of glycine betaine synthesis genes linked by the FMDV 2A region in a single open reading frame in Pichia pastoris. Wang, Sanhong,Yao, Quanhong,Tao, Jianmin,Qiao, Yushan,Zhang, Zhen.

[13]Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast. Sun, M,Qian, KX,Su, N,Chang, HY,Liu, JX,Chen, GF.

[14]A Novel miRNA Sponge Form Efficiently Inhibits the Activity ofmiR393 and Enhances the Salt Tolerance and ABA Insensitivity in Arabidopsis thaliana. Long, Ruicai,Li, Xiao,Gao, Yanli,Zhang, Tiejun,Kang, Junmei,Cong, Lili,Yang, Qingchuan,Li, Mingna,Sun, Yan,Wang, Tenghua.

[15]Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines. Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian,Scott, Nigel W.,Elliott, Malcolm C.,Slater, Adrian,Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian.

[16]Isolation and characterization of a harvest-induced promoter of an alfalfa gene, hi7. Zhang, Jian,Yu, Wen-Jin,Bahramnejad, Bahman,Erickson, Larry R.,Xiong, Ai-Sheng,Bahramnejad, Bahman,Yu, Wen-Jin,Erickson, Larry R..

[17]Comparative flight performance of three important pest Adelphocoris species of Bt cotton in China. Wu, K. M.,Guo, Y. Y.,Wyckhuys, K. A. G..

[18]Interactions of elevated carbon dioxide and temperature with aphid feeding on transgenic oilseed rape: Are Bacillus thuringiensis (Bt) plants more susceptible to nontarget herbivores in future climate?. Himanen, Sari J.,Nissinen, Anne,Nerg, Anne-Marja,Holopainen, Jarmo K.,Nissinen, Anne,Dong, Wen-Xia,Stewart, C. Neal, Jr.,Poppy, Guy M..

[19]Isolation and functional characterization of HvDREB1-a gene encoding a dehydration-responsive element binding protein in Hordeum vulgare. Xu, Zhao-Shi,Ni, Zhi-Yong,Li, Zhi-Yong,Li, Lian-Cheng,Chen, Ming,Gao, Dong-Yao,Yu, Xiu-Dao,Liu, Pei,Ma, You-Zhi.

[20]Directed evolution of a beta-galactosidase from Pyrococcus woesei resulting in increased thermostable beta-glucuronidase activity. Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Li, Xian,Xue, Yong,Liu, Jin-Ge,Gao, Feng,Cai, Bin,Chen, Jian-Min,Yao, Quan-Hong.

作者其他论文 更多>>

微信小程序