Pistil drip following pollination: a simple in planta Agrobacterium-mediated transformation in cotton

文献类型： 外文期刊

第一作者： Chen TianZi

作者： Chen TianZi;Wu ShenJie;Zhao Jun;Guo WangZhen;Zhang TianZhen;Wu ShenJie

作者机构：

关键词： cotton;genetic engineering;genetic transformation;genomes;herbicide resistance;heritability;transgenic plants;Gossypium

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

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收录情况： SCI

摘要： Transgenic cotton plants were developed by pistil drip inoculation in a solution containing Agrobacterium carrying a gene for resistance to the herbicide Basta (bar), 10% (w/v) sucrose, 0.05% (v/v) Silwet L-77 and 40 mg acetosyringone lp#. Pistil drip during 17:00-19:00 on the first day of flowering resulted in 0.07-0.17% Basta-resistant plants/number of viable seeds generated, and stigma excision prior to pistil drip during this time period gave rise to a transformation efficiency of 0.46-0.93%, in contrast with 0.04-0.06% generated from pistil drip during 9:00-11:00 on the second day of flowering. PCR and Southern blot analysis confirmed the integration of the bar gene into the cotton genome, and a T1 and T2 generation herbicide resistance test consistently revealed expression and stable heritability of the bar gene in the two generations.

分类号： Q7

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