Prime-boost immunization with HA/C3d DNA followed by a recombinant pseudorabies virus boost enhanced protective immunity against H3N2 swine influenza virus in mice
文献类型: 外文期刊
第一作者: Li, Guo-Xin
作者: Li, Guo-Xin;Zhou, Yan-Jun;Yu, Hai;Yan, Li-Ping;Tong, Guang-Zhi;Li, Guo-Xin;Tian, Zhi-Jun;Hu, Shou-Ping;Tong, Guang-Zhi;Zhang, Qiang;Zhang, Qiang
作者机构:
关键词: immune response;virus neutralization;vaccine regimen;protective humoral immunity;immunogenicity induction
期刊名称:RESEARCH IN VETERINARY SCIENCE ( 影响因子:2.534; 五年影响因子:2.382 )
ISSN:
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收录情况: SCI
摘要: DNA and recombinant virus vaccines against swine influenza virus (SIV) have been pursued with promising results, but induce poor immunogenicity. This study evaluated the effects of a vaccine regimen in mice including priming with three DNA vaccines expressing soluble HA (sHA), complete HA (tmHA), or sHA fused with three copies murine C3d (5HA-mC3d3) and boosting with recombinant pseudorabies virus expressing HA (rPRV-HA). Immune responses were monitored by ELISA, HI assays, and virus neutralization. Protective efficacy was evaluated by virus isolation from lungs, distribution in tissues, and pathology following challenge with H3N2 Sly. Priming with sHA-mC3d3 and boosting with rPRV-HA induced higher levels of HA-specific antibodies and yielded the most effective protection. This finding implied that priming with a DNA vaccine expressing C3d fused with antigen and boosting with a recombinant vector vaccine is an effective way to induce protective humoral immunity and prevent some infectious diseases.
分类号: S85
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