ClZISO mutation leads to photosensitive flesh in watermelon

文献类型： 外文期刊

第一作者： Zhang, Jie

作者： Zhang, Jie;Sun, Honghe;Guo, Shaogui;Ren, Yi;Li, Maoying;Wang, Jinfang;Yu, Yongtao;Zhang, Haiying;Gong, Guoyi;He, Hongju;Zhang, Chao;Xu, Yong

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.574； 五年影响因子：5.662 ）

ISSN： 0040-5752

年卷期： 2022 年 135 卷 5 期

页码：

收录情况： SCI

摘要： The mutation of ClZISO identified in EMS-induced watermelon leads to photosensitive flesh in watermelon. Watermelon (Citrullus lanatus) has a colorful flesh that attracts consumers and benefits human health. We developed an ethyl-methanesulfonate mutation library in red-fleshed line '302' to create new flesh color lines and found a yellow-fleshed mutant which accumulated zeta-carotene. The initial yellow color of this mutant can be photobleached within 10 min under intense sunlight. A long-term light-emitting diode (LED) light treatment turned flesh color from yellow to pink. We identified this unique variation as photosensitive flesh mutant ('psf'). Using bulked segregant analysis, we fine-mapped an EMS-induced G-A transversion in 'psf' which leads to a premature stop codon in 15-cis-zeta-carotene isomerase (ClZISO) gene. We detected that wild-type ClZISO is expressed in chromoplasts to catalyze the conversion of 9,15,9'-tri-cis-zeta-carotene to 9,9'-di-cis-zeta-carotene. The truncated ClZISO(mu) protein in psf lost this catalytic function. Light treatment can partially compensate ClZISO(mu) isomerase activity via photoisomerization in vitro and in vivo. Transcriptome analysis showed that most carotenoid biosynthesis genes in psf were downregulated. The dramatic increase of ABA content in flesh with fruit development was blocked in psf. This study explores the molecular mechanism of carotenoid biosynthesis in watermelon and provides a theoretical and technical basis for breeding different flesh color lines in watermelon.

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