Rapid and direct detection of m6A methylation by DNAzyme-based and smartphone-assisted electrochemical biosensor

文献类型： 外文期刊

第一作者： Li, Kai

作者： Li, Kai;Liang, Yu;Li, Xinran;Yang, Mengrui;Wang, Min;Li, Fukai;Qi, Xin;Zhou, Jian;Li, Liang;Fu, Wei

作者机构：

关键词： DNAzyme; Tetrahedron DNA; Electrochemical biosensor; m(6)A methylation

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.5； 五年影响因子：10.1 ）

ISSN： 0956-5663

年卷期： 2025 年 267 卷

页码：

收录情况： SCI

摘要： m(6)A methylation detection is crucial for understanding RNA functions, revealing disease mechanisms, guiding drug development and advancing epigenetics research. Nevertheless, high-throughput sequencing and liquid chromatography-based traditional methods still face challenges to rapid and direct detection of m(6)A methylation. Here we report a DNAzyme-based and smartphone-assisted electrochemical biosensor for rapid detection of m(6)A. We initially identified m(6)A methylation-sensitive DNAzyme mutants through site mutation screening. These mutants were then combined with tetrahedral DNA to modify the electrodes, creating a 3D sensing interface. The detection of m(6)A was accomplished by using DNAzyme to capture and cleave the m(6)A sequence. The electrochemical biosensor detected the m(6)A sequence at nanomolar concentrations with a low detection limit of 0.69 nM and a wide detection range from 10 to 10(4) nM within 60 min. As a proof of concept, the 3 '-UTR sequence of rice was selected as the m(6)A analyte. Combined with a smartphone, our biosensor shows good specificity, sensitivity, and easy-to-perform features, which indicates great prospects in the field of RNA modification detection and epigenetic analysis.

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