Genetic Mechanism of Tissue-Specific Expression of PPAR Genes in Turbot (Scophthalmus maximus) at Different Temperatures

文献类型： 外文期刊

第一作者： Wang, Xinan

作者： Wang, Xinan;Ma, Aijun;Wang, Xinan;Ma, Aijun;Zhao, Tingting

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关键词： genotype; tissue; interactions; PPAR genes; turbot Scophthalmus maximus

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

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年卷期： 2022 年 23 卷 20 期

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收录情况： SCI

摘要： In this study, we used PCR to measure the levels of the peroxisome proliferator activated receptor genes PPAR alpha 1, PPAR alpha 2, PPAR beta, and PPAR gamma in the intestine, liver, gill, heart, kidney, brain, muscle, spleen, skin, and stomach of turbot (Scophthalmus maximus) cultured under different temperature conditions (14, 20, 23, 25, and 28 degrees C). We used split-split-plot (SSP) analysis of variance, additive main effects and multiplicative interaction (AMMI) analysis, and genotype main effects and genotype x environment interaction (GGE) biplot analysis to evaluate the genotype x tissue interaction effects on gene expression. The results of the SSP analysis of variance showed that temperature and tissue x gene have highly significant (p < 0.01) effect on the expression of S. maximus PPAR genes. The AMMI analysis results revealed that the expression of PPAR genes at the appropriate temperature (14 degrees C) mainly depended on genotype x tissue interaction and tissue effects. Under stress temperatures, genotype effects, tissue effects, and genotype x tissue interaction, all had significant effects on the expression of PPAR genes. The contribution of the genotype effect slowly increased with increasing temperature; it increased faster at 20 degrees C and then slowly declined at 25 degrees C. The contribution of the tissue effect slowly increased from 14 to 20 degrees C, where it sharply decreased, and then it stabilized after a slight fluctuation. The contribution of the genotype x tissue interaction effect showed a fluctuating upward trend throughout the experiment, and it had a significant impact on PPAR gene expression. The key temperature at which the three effects changed was 20 degrees C, indicating that it is the limit temperature for active lipid metabolism under high-temperature stress. The GGE biplot analysis results showed that under suitable water temperature, the expression difference of PPAR genes in the liver was the largest; at 20 and 23 degrees C, the expression difference in the gill was the largest; and at 25 and 28 degrees C, the expression difference in the brain was the largest. Overall, our results suggest that the mechanism responsible for PPAR gene expression under the three high temperatures (23, 25, and 28 degrees C) was relatively consistent, but it differed from that at 20 degrees C.

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