DNA enrichment using deep eutectic solvents for improved PCR detection in Antarctic krill oil

文献类型： 外文期刊

第一作者： Su, Dong

作者： Su, Dong;Ma, Xinyu;Miao, Junkui;Liu, Xiaofang;Leng, Kailiang;Wang, Xixi;Su, Dong;Ma, Xinyu;Zhang, Yating;Zhao, Ling;Yu, Yueqin;Su, Dong;Su, Dong;Leng, Kailiang

作者机构：

关键词： Euphausia superba; Krill oil; Deep eutectic solvents; 16S rRNA; Food authenticity

期刊名称：SEPARATION AND PURIFICATION TECHNOLOGY （ 影响因子：9.0； 五年影响因子：8.5 ）

ISSN： 1383-5866

年卷期： 2025 年 374 卷

页码：

收录情况： SCI

摘要： The commercialization of Antarctic krill oil raises concerns about product authenticity and adulteration. In this study, we developed a DNA-based authentication method for Antarctic krill oil. Species-specific PCR primers targeting a 157-bp fragment of the Euphausia superba 16S rRNA gene were designed, enabling high-specificity differentiation from Thunnus alalunga, Gadus ogac, and Salmo salar. The optimized PCR method achieved a detection sensitivity of 0.1 ng DNA/reaction. To address challenges in DNA extraction from complex lipid matrices, n-hexane and choline-based deep eutectic solvents (DES) were screened for their ability to separate interferents and enrich DNA. Among these, choline chloride-formic acid was selected as the optimal solvent due to its broader positive electrostatic potential (ESP) distribution (108.7 & Aring;(2), 56.0%). Post-PCR analysis successfully distinguished Antarctic krill oil from other edible oils. This work provides a robust tool for protecting high-value oils and advancing authenticity verification in related products.

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