Characterization of a novel bifunctional enzyme from buffalo rumen metagenome and its effect on in vitro ruminal fermentation and microbial community composition

文献类型： 外文期刊

第一作者： Meng, Zhenxiang

作者： Meng, Zhenxiang;Ma, Jing;Sun, Zhanying;Zhu, Weiyun;Cheng, Yanfen;Cheng, Yanfen;Yang, Chengjian;Leng, Jing

作者机构：

关键词： Bifunctional enzyme; In vitro ruminal fermentation; Lignocellulose; Microbial community

期刊名称：ANIMAL NUTRITION （ 影响因子：6.3； 五年影响因子：6.9 ）

ISSN： 2405-6383

年卷期： 2023 年 13 卷

页码：

收录情况： SCI

摘要： To efficiently use lignocellulosic materials in ruminants, it is crucial to explore effective enzymes, especially bifunctional enzymes. In this study, a novel stable bifunctional cellulase-xylanase protein from buffalo rumen metagenome was expressed and characterized, CelXyn2. The enzyme displayed optimal activity at pH 6.0 and 45 degrees C. The residual endoglucanase and xylanase activities were 90.6% and 86.4% after a 60-min pre-incubation at 55 degrees C. Hydrolysis of rice straw, wheat straw, sheepgrass and sugar beet pulp by CelXyn2 showed its ability to degrade both cellulose and hemicellulose polymers. Treatment with CelXyn2 improved the hydrolysis of agricultural residues with an evident increase in production of total gas, lactate and volatile fatty acids. The results of 16S rRNA and real- time PCR showed that the effect on in vitro ruminal microbial community depended on fermenta- tion substrates. This study demonstrated that CelXyn2 could strengthen lignocellulose hydrolysis and in vitro ruminal fermentation. These characteristics of CelXyn2 distinguish it as a promising candidate for agricultural application.(c) 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by- nc-nd/4.0/).

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