Hollow Hierarchical Porous and Antihydrolytic Spherical Zeolitic Imidazolate Frameworks for Enzyme Encapsulation and Biocatalysis

文献类型： 外文期刊

第一作者： Zhao, Baozhu

作者： Zhao, Baozhu;Yang, Haowen;Zheng, Lei;Shi, Jie;Mao, Jin;Zhou, Qi;Deng, Qianchun

作者机构：

关键词： metal-organic frameworks; hollow hierarchicalporous; water intolerance; immobilized enzyme; phytosterol esters

期刊名称：ACS APPLIED MATERIALS & INTERFACES （ 影响因子：9.5； 五年影响因子：9.6 ）

ISSN： 1944-8244

年卷期： 2024 年 16 卷 7 期

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收录情况： SCI

摘要： The creation of a new metal-organic framework (MOF) with a hollow hierarchical porous structure has gained significant attention in the realm of enzyme immobilization. The present work employed a novel, facile, and effective combinatorial technique to synthesize modified MOF (N-PVP/HZIF-8) with a hierarchically porous core-shell structure, allowing for the preservation of the structural integrity of the encapsulated enzyme molecules. Scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, confocal laser scanning microscopy, and other characterization tools were used to fully explore the changes of morphological structure and surface properties in different stages of the preparation of immobilization enzyme CRL-N-PVP/HZIF-8, thus showing the superiority of N-PVP/HZIF-8 as an enzyme immobilization platform and the logic of the immobilization process on the carrier. Additionally, the maximum enzyme loading was 216.3 mg mL(-1), the relative activity of CRL-N-PVP/HZIF-8 increased by 15 times compared with the CRL@ZIF-8 immobilized in situ, and exhibited quite good thermal, chemical, and operational stability. With a maximal conversion of 88.8%, CRL-N-PVP/HZIF-8 demonstrated good catalytic performance in the biosynthesis of phytosterol esters as a proof of concept. It is anticipated that this work will offer fresh concepts from several perspectives for the creation of MOF-based immobilized enzymes for biotechnological uses.

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