Single-cell genetic models to evaluate orphan gene function: The case of QQS regulating carbon and nitrogen allocation

文献类型： 外文期刊

第一作者： Wang, Lei

作者： Wang, Lei;Tanvir, Rezwan;Zhang, Jinjiang;Cothron, Samuel;Li, Ling;Tonsager, Andrew J.;Stenback, Kenna E.;Campbell, Alexis;Nikolau, Basil J.;Tonsager, Andrew J.;Stenback, Kenna E.;Campbell, Alexis;Nikolau, Basil J.;Tonsager, Andrew J.;Stenback, Kenna E.;Campbell, Alexis;Nikolau, Basil J.;Zheng, Wenguang;Wang, Yingjun;Stessman, Dan;Fang, Wei;Spalding, Martin H.;Zhang, Jinjiang;Wan, Dongli;Meng, Yan

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关键词： single-cell systems; orphan gene QQS; NF-YC homologs; HAP2; HAP3; HAP5; carbon and nitrogen partitioning; Chlamydomonas reinhardtii and Saccharomyces cerevisiae

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： We demonstrate two synthetic single-cell systems that can be used to better understand how the acquisition of an orphan gene can affect complex phenotypes. The Arabidopsis orphan gene, Qua-Quine Starch (QQS) has been identified as a regulator of carbon (C) and nitrogen (N) partitioning across multiple plant species. QQS modulates this important biotechnological trait by replacing NF-YB (Nuclear Factor Y, subunit B) in its interaction with NF-YC. In this study, we expand on these prior findings by developing Chlamydomonas reinhardtii and Saccharomyces cerevisiae strains, to refactor the functional interactions between QQS and NF-Y subunits to affect modulations in C and N allocation. Expression of QQS in C. reinhardtii modulates C (i.e., starch) and N (i.e., protein) allocation by affecting interactions between NF-YC and NF-YB subunits. Studies in S. cerevisiae revealed similar functional interactions between QQS and the NF-YC homolog (HAP5), modulating C (i.e., glycogen) and N (i.e., protein) allocation. However, in S. cerevisiae both the NF-YA (HAP2) and NF-YB (HAP3) homologs appear to have redundant functions to enable QQS and HAP5 to affect C and N allocation. The genetically tractable systems that developed herein exhibit the plasticity to modulate highly complex phenotypes.

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