A P450 superfamily member NtCYP82C4 promotes nicotine biosynthesis in Nicotiana tabacum

文献类型： 外文期刊

第一作者： Zeng, Wanli

作者： Zeng, Wanli;Kong, Weisong;Song, Chunman;Huang, Haitao;Deng, Lele;Gao, Qian;Wang, Kunmiao;Xiang, Haiying;Shi, Chuhan;Meng, Yang;Xu, Fangzheng;Cui, Mengmeng;Ning, Yang;Wang, Qian;Shi, Chuhan;Meng, Yang

作者机构：

关键词： Nicotine biosynthesis; P450; Tobacco

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：2.2； 五年影响因子：2.5 ）

ISSN： 0006-291X

年卷期： 2024 年 739 卷

页码：

收录情况： SCI

摘要： In plants, cytochrome P450s are monooxygenase that play key roles in the synthesis and degradation of intracellular substances. In tobacco, the majority of studies examining the P450 superfamily have concentrated on the CYP82E subfamily, where multiple family members function as demethylases, facilitating the synthesis of nor- nicotine. In this study, NtCYP82C4, a tobacco P450 superfamily member, was identified from a gene-edited tobacco mutant that nicotine biosynthesis in tobacco leaves is evidently reduced. Compared to the wild-type plants, the knockout of NtCYP82C4 resulted in a significantly lower nicotine content and biomass in tobacco leaves. Transcriptome and metabolome analyses indicated that the knockout of NtCYP82C4 inhibites secondary metabolic processes in tobacco plants, leading to the accumulation of some important precursors in the nicotine synthesis process, including aspartic acid and nicotinic acid, and increases nitrogen partitioning associated with those processes such as amino acid synthesis and utilization. It is speculated that NtCYP82C4 may function as an important catalase downstream of the nicotine synthesis. Currently, most of the steps and enzymes involved in the nicotine biosynthesis process in tobacco have been elucidated. Here, our study deepens the current understanding of nicotine biosynthesis process and provides new enzyme targets for nicotine synthesis in tobacco plants.

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